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dc.contributor.author
Rodriguez, Juan M.
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Marchicio, José
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López, Mariela
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Ziblat, Andrea
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Elias, Fernanda
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Fló, Juan
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López, Ricardo A.
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Horn, David
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Zorzopulos, Jorge
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Montaner, Alejandro Daniel
dc.date.available
2016-12-05T19:01:03Z
dc.date.issued
2015-02-23
dc.identifier.citation
Rodriguez, Juan M.; Marchicio, José; López, Mariela; Ziblat, Andrea; Elias, Fernanda; et al.; PyNTTTTGT and CpG immunostimulatory oligonucleotides: effect on granulocyte/monocyte colony-stimulating factor (GM-CSF) secretion by human CD56+ (NK and NKT) cells; Public Library Of Science; Plos One; 10; 2; 23-2-2015; e0117484.-e0117484
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/8798
dc.description.abstract
CD56+ cells have been recognized as being involved in bridging the innate and acquired immune systems. Herein, we assessed the effect of two major classes of immunostimulatory oligonucleotides (ODNs), PyNTTTTGT and CpG, on CD56+ cells. Incubation of human peripheral blood mononuclear cells (hPBMC) with some of these ODNs led to secretion of significant amounts of interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α) and granulocyte/monocyte colony-stimulating factor (GM-CSF), but only if interleukin 2 (IL2) was present. IMT504, the prototype of the PyNTTTTGT ODN class, was the most active. GM-CSF secretion was very efficient when non-CpG ODNs with high T content and PyNTTTTGT motifs lacking CpGs were used. On the other hand, CpG ODNs and IFNα inhibited this GM-CSF secretion. Selective cell type removal from hPBMC indicated that CD56+ cells were responsible for GM-CSF secretion and that plasmacytoid dendritic cells (PDCs) regulate this process. In addition, PyNTTTTGT ODNs inhibited the IFNα secretion induced by CpG ODNs in PDCs by interference with the TLR9 signaling pathway. Since IFNα is essential for CD56+ stimulation by CpG ODNs, there is a reciprocal interference of CpG and PyNTTTTGT ODNs when acting on this cell population. This suggests that these synthetic ODNs mimic different natural alarm signals for activation of the immune system.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Oligonucleotides
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Cpg
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Gm-Csf
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Cd56 Cells
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Inmunología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
PyNTTTTGT and CpG immunostimulatory oligonucleotides: effect on granulocyte/monocyte colony-stimulating factor (GM-CSF) secretion by human CD56+ (NK and NKT) cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
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info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-10-04T19:26:40Z
dc.identifier.eissn
1932-6203
dc.journal.volume
10
dc.journal.number
2
dc.journal.pagination
e0117484.-e0117484
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Rodriguez, Juan M.. Fundacion Pablo Cassara; Argentina
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Fil: Marchicio, José. Immunotech S.a.. Departamento de Investigacion y Desarrollo; Argentina
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Fil: López, Mariela. Immunotech S.a.. Departamento de Investigacion y Desarrollo; Argentina
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Fil: Ziblat, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Immunotech S.a.; Argentina
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Fil: Elias, Fernanda. Immunotech S.a.. Departamento de Investigacion y Desarrollo; Argentina
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Fil: Fló, Juan. Immunotech S.a.. Departamento de Investigacion y Desarrollo; Argentina
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Fil: López, Ricardo A.. Immunotech S.a.. Departamento de Investigacion y Desarrollo; Argentina
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Fil: Horn, David. David Horn LLC; Estados Unidos
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Fil: Zorzopulos, Jorge. Immunotech S.a.. Departamento de Investigacion y Desarrollo; Argentina
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Fil: Montaner, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0117484
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info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0117484
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338301/