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dc.contributor.author
Pierre, Alice
dc.contributor.author
Racine, Chrystèle
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Rey, Rodolfo Alberto
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dc.contributor.author
Fanchin, Renato
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Taieb, Joëlle
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Cohen Tannoudji, Joëlle
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Carmillo, Paul
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Pepinsky, R. Blake
dc.contributor.author
Cate, Richard L.
dc.contributor.author
Di Clemente, Nathalie
dc.date.available
2019-10-16T21:28:29Z
dc.date.issued
2016-12
dc.identifier.citation
Pierre, Alice; Racine, Chrystèle; Rey, Rodolfo Alberto; Fanchin, Renato; Taieb, Joëlle; et al.; Most cleaved anti-müllerian hormone binds its receptor in human follicular fluid but little is competent in serum; Endocrine Society; Journal of Clinical Endocrinology and Metabolism; 101; 12; 12-2016; 4618-4627
dc.identifier.issn
0021-972X
dc.identifier.uri
http://hdl.handle.net/11336/86111
dc.description.abstract
Context: Anti-Müllerian hormone (AMH) is an important clinical marker for diagnosing and assessing the reproductive status and/or disorders in men and women. Most studies have not distinguished between levels of inactive AMH precursor and the cleaved noncovalent complex that binds the AMH type II receptor (AMHRII) and initiates signaling. Objective: The objective of the study was to measure the levels of AMH cleavage and bioactivity in human body fluids. Design, Setting, and Patients: AMH cleavage levels and bioactivity were measured in the serum of six boys and in the follicular fluid and serum of nine control women and 13 women with the polycystic ovary syndrome (PCOS). Main Outcome Measures: AMH cleavage levels were measured by capturing AMH with an anti- AMH antibody, followed by Western blotting. The bioactivity of cleaved AMH was assessed with an ELISA that measures the levels of AMH capable of binding AMHRII. Results: PCOS women have an elevated level of AMH cleavage in their follicular fluid (24% vs 8% in controlwomen),andmostofthecleavedAMHcanbindAMHRII.Higherlevels of cleavage areobserved in female (60%) and male (79%) serum, but very little of the cleaved AMH can bind AMHRII. Conclusions: These results support an autocrine role for AMH in the pathophysiology of PCOS in the follicle. In addition,theyindicate thatAMHundergoesinteractions or structuralchangesaftercleavage that prevent receptor binding, meaning, unexpectedly, that the level of cleaved AMH in biological fluids does not always reflect the level of bioactive AMH.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Endocrine Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AMH
dc.subject
Cleavage
dc.subject
PCOS
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testis
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Most cleaved anti-müllerian hormone binds its receptor in human follicular fluid but little is competent in serum
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-02T14:27:40Z
dc.journal.volume
101
dc.journal.number
12
dc.journal.pagination
4618-4627
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington DC
dc.description.fil
Fil: Pierre, Alice. Inserm; Francia. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia
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Fil: Racine, Chrystèle. Inserm; Francia. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia
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Fil: Rey, Rodolfo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina
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Fil: Fanchin, Renato. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia
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Fil: Taieb, Joëlle. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia
dc.description.fil
Fil: Cohen Tannoudji, Joëlle. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia
dc.description.fil
Fil: Carmillo, Paul. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil
Fil: Pepinsky, R. Blake. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil
Fil: Cate, Richard L.. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia
dc.description.fil
Fil: Di Clemente, Nathalie. Inserm; Francia. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia
dc.journal.title
Journal of Clinical Endocrinology and Metabolism
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1210/jc.2016-1742
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jcem/article/101/12/4618/2765023
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