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Artículo

Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk

Pena, Gabriela AlejandraIcon ; Alonso, Veronica AndreaIcon ; Velasco Manini, Marina AndreaIcon ; Pellegrino, Matias SantiagoIcon ; Cavaglieri, Lilia ReneéIcon
Fecha de publicación: 06/2018
Editorial: Elsevier Science
Revista: International Journal of Food Microbiology
ISSN: 0168-1605
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Micología

Resumen

Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a gliotoxinogenic species into section Fumigati commonly found in contaminated animal environments. In dairy herds, exposed areas of lactating cows, as mammalian glandule, can be easily contaminated by them. This study was aimed to identify A. fumigatus sensu lato strains (identified based on morphology) isolated from raw cow milk at species level, by morphological and molecular techniques, and to estimate their genetic variability. Forty-five A. fumigatus strains showed similar RAPD profiles (generated with PELF and URP1F primers) to each other and to A. fumigatus sensu stricto reference strains; also, they were almost identical to clinical human and feed-borne A. fumigatus strains included in the assay, since their similarity coefficient ranged from 0.7 to 1.00. Therefore, all strains were characterized as belonging to A. fumigatus sensu stricto species. This result was supported by sequencing the benA gene of selected strains and by maximum parsimony analysis. In addition, RAPD fingerprinting demonstrated intra-specific genetic variability into the A. fumigatus sensu stricto cluster. The results found in this study strengthen the fact that A. fumigatus sensu stricto is the predominant species in the Aspergillus section Fumigati found in animal environments such as dairy herd environments, while other species such as A. novofumigatus, A. fumigatiaffinis, A. udagawae and A. lentulus may be rarely isolated. Since no differences between animal and human strains were observed they can become pathogenic also for farm handlers’. Moreover, the presence of A. fumigatus sensu stricto in raw cow milk is probably a very important risk factor since milk and its by-products are generally indented for human consumption, then gliotoxin could be transferred to them.
Palabras clave: A. FUMIGATUS SENSU STRICTO , GENETIC VARIABILITY ESTIMATION , MOLECULAR TYPING , RAW COW MILK
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/85517
URL: http://linkinghub.elsevier.com/retrieve/pii/S0168160518301211
DOI: http://dx.doi.org/10.1016/j.ijfoodmicro.2018.03.018
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Citación
Pena, Gabriela Alejandra; Alonso, Veronica Andrea; Velasco Manini, Marina Andrea; Pellegrino, Matias Santiago; Cavaglieri, Lilia Reneé; Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk; Elsevier Science; International Journal of Food Microbiology; 275; 6-2018; 1-7
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