Artículo
Variability in gut mucosal secretory IgA in mice along a working day
Burns, Patricia Graciela
; Oddi, Sofia Lorena
; Forzani, Liliana Maria
; Tabacman, Eduardo; Reinheimer, Jorge Alberto
; Vinderola, Celso Gabriel
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Fecha de publicación:
02/2018
Editorial:
BioMed Central Ltd.
Revista:
BMC Research Notes
ISSN:
1756-0500
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Objective: To assess the variability of secretory immunoglobulin A (S-IgA) in the lumen and feces of mice along a working day. Results: Mice were maintained under a 12 h light-dark cycle, light period starting at 8 AM. S-IgA was determined in feces and intestinal content (after one or three washes) at three points along the day: at the beginning, in the middle and at the end of the light period (ELP). Significant reduction in the content of S-IgA in the small intestine fluid and in feces was observed at the end of the light cycle, which coincides with the end of a regular working day (8 PM) in any given animal facility. It was also observed that three washes of the small intestine were more effective than one flush to recover a significant higher amount of S-IgA, with the smallest coefficient of variation observed by the ELP. A smaller CV would imply a reduced number of animals needed to achieve the same meaningful results. The results may be useful when designing animal trials for the selection of probiotic candidates based on their capacity of activating S-IgA, since it would imply a more rational use of experimental animals.
Palabras clave:
CIRCADIAN RHYTHM
,
IGA
,
INTESTINE
,
MICE
,
VARIABILITY
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Licencia
Identificadores
Colecciones
Articulos(INLAIN)
Articulos de INST.DE LACTOLOGIA INDUSTRIAL
Articulos de INST.DE LACTOLOGIA INDUSTRIAL
Citación
Burns, Patricia Graciela; Oddi, Sofia Lorena; Forzani, Liliana Maria; Tabacman, Eduardo; Reinheimer, Jorge Alberto; et al.; Variability in gut mucosal secretory IgA in mice along a working day; BioMed Central Ltd.; BMC Research Notes; 11; 1; 2-2018; 1-5
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