Artículo
Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii
Fecha de publicación:
05/2002
Editorial:
Elsevier Science Inc
Revista:
Enzyme and Microbial Technology
ISSN:
0141-0229
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We report the efficient enantioselective reduction of a number of β-ketoesters by mycelial and yeast-like forms of the dimorphic fungus Mucor rouxii in a whole-cell process. Mycelial cells, grown in aerobiosis, were efficient in water, whereas the yeast-like cells, grown in anaerobic medium, were both efficient in water and in organic solvents. Almost 100% of conversion with 97% of enantiomeric excess of the (S) forms of the reduced β-ketoesters was obtained at 15 g biomass (wet weight)/mmol substrate in two hours. The fungal spores, which are the physiologically resistant form of the fungus, also catalyzed the reductive process efficiently and stereoselectively. The freeze-dried as well as the warm-air dried yeast-like cells, rehydrated in a small volume of water, maintained the same efficiency and selectivity of the reaction in organic solvents as the fresh biomass up to at least 4 months.
Palabras clave:
Β-KETOESTERS
,
MUCOR ROUXII
,
MYCELIAL
,
SPORES
,
YEAST
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Identificadores
Colecciones
Articulos(UMYMFOR)
Articulos de UNID.MICROANAL.Y MET.FISICOS EN QUIM.ORG.(I)
Articulos de UNID.MICROANAL.Y MET.FISICOS EN QUIM.ORG.(I)
Citación
Mangone, Constanza P; Pereyra, Elba Nora; Argimón, Silvia; Moreno, Silvia; Baldessari, Alicia; Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii; Elsevier Science Inc; Enzyme and Microbial Technology; 30; 5; 5-2002; 596-601
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