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dc.contributor.author
Lottersberger, Javier
dc.contributor.author
Guerrero, Sergio Adrian
dc.contributor.author
Tonarelli, Georgina Guadalupe
dc.contributor.author
Frank, R.
dc.contributor.author
Tarabla, Hector Dante
dc.contributor.author
Vanasco, Norma Bibiana
dc.date.available
2019-09-27T19:09:51Z
dc.date.issued
2009
dc.identifier.citation
Lottersberger, Javier; Guerrero, Sergio Adrian; Tonarelli, Georgina Guadalupe; Frank, R.; Tarabla, Hector Dante; et al.; Epitope mapping of pathogenic leptospira LipL32; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 49; 5; 2009; 41-45
dc.identifier.issn
0266-8254
dc.identifier.uri
http://hdl.handle.net/11336/84710
dc.description.abstract
Aims: To identify LipL32 epitopes and to evaluate their capability to recognizespecific antibodies using ELISA.Methods and Results: Epitope mapping by means of a library of overlappingpeptide fragments prepared by simultaneous and parallel solid phase peptidesynthesis on derivatized cellulose membranes (SPOT synthesis) was carried out.Eighty-seven overlapping decapentapeptides corresponding to the completesequence of LipL32 were synthesized. According to spot-image intensities, themost reactive sequences were localized in regions 151?177 (sequenceAAKAKPVQKLDDDDDGDDTYKEERHNK) and 181?204 (sequence LTRIKIPNPPKSFDDLKNIDTKKL).Two peptides (P1 and P2) corresponding tothese sequences were synthesized, and their reactivity evaluated using ELISAtest.Conclusions: Epitope identification and analysis suggested the existence of twoantigenic regions within LipL32. These LipL32 reactive regions were highlyconserved among antigenically variants of Leptospira spp. isolates. Peptidescontaining these regions (P1 and P2) showed a good capability for anti-leptospiralantibody recognition.Significance and Impact of the Study: This finding could have potential relevancenot only for serodiagnosis but also as a starting point for the characterizationof targets for vaccine design.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
EPITOPE
dc.subject
LEPTOSPIRA
dc.subject
LIPLP32
dc.subject
MAPPING
dc.subject.classification
Biotecnología relacionada con la Salud
dc.subject.classification
Biotecnología de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Epitope mapping of pathogenic leptospira LipL32
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-09-27T14:16:22Z
dc.journal.volume
49
dc.journal.number
5
dc.journal.pagination
41-45
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Lottersberger, Javier. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
dc.description.fil
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
dc.description.fil
Fil: Frank, R.. Helmholtz Centre for Environmental Research; Alemania
dc.description.fil
Fil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Vanasco, Norma Bibiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Respiratorias; Argentina
dc.journal.title
Letters in Applied Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2009.02723.x/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1472-765X.2009.02723.x/abstract


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