Artículo
Epitope mapping of pathogenic leptospira LipL32
Lottersberger, Javier; Guerrero, Sergio Adrian
; Tonarelli, Georgina Guadalupe; Frank, R.; Tarabla, Hector Dante; Vanasco, Norma Bibiana
Fecha de publicación:
2009
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Letters in Applied Microbiology
ISSN:
0266-8254
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Aims: To identify LipL32 epitopes and to evaluate their capability to recognizespecific antibodies using ELISA.Methods and Results: Epitope mapping by means of a library of overlappingpeptide fragments prepared by simultaneous and parallel solid phase peptidesynthesis on derivatized cellulose membranes (SPOT synthesis) was carried out.Eighty-seven overlapping decapentapeptides corresponding to the completesequence of LipL32 were synthesized. According to spot-image intensities, themost reactive sequences were localized in regions 151?177 (sequenceAAKAKPVQKLDDDDDGDDTYKEERHNK) and 181?204 (sequence LTRIKIPNPPKSFDDLKNIDTKKL).Two peptides (P1 and P2) corresponding tothese sequences were synthesized, and their reactivity evaluated using ELISAtest.Conclusions: Epitope identification and analysis suggested the existence of twoantigenic regions within LipL32. These LipL32 reactive regions were highlyconserved among antigenically variants of Leptospira spp. isolates. Peptidescontaining these regions (P1 and P2) showed a good capability for anti-leptospiralantibody recognition.Significance and Impact of the Study: This finding could have potential relevancenot only for serodiagnosis but also as a starting point for the characterizationof targets for vaccine design.
Palabras clave:
EPITOPE
,
LEPTOSPIRA
,
LIPLP32
,
MAPPING
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Articulos(IAL)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Citación
Lottersberger, Javier; Guerrero, Sergio Adrian; Tonarelli, Georgina Guadalupe; Frank, R.; Tarabla, Hector Dante; et al.; Epitope mapping of pathogenic leptospira LipL32; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 49; 5; 2009; 41-45
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