Artículo
Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats
Vignola, María Belén
; Dávila, Soledad; Cremonezzi, David; Simes, Juan Carlos; Palma, José A.; Campana, Vilma
Fecha de publicación:
19/12/2012
Editorial:
Informa Healthcare
Revista:
Electromagnetic Biology And Medicine
ISSN:
1536-8378
e-ISSN:
1536-8386
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The effect of pulsed electromagnetic field (PEMF) therapy, also called magnetic therapy, upon inflammatory biomarkers associated with oxidative stress plasma fibrinogen, nitric oxide (NO), L-citrulline, carbonyl groups, and superoxide dismutase (SOD) was evaluated through histological assessment, in rats with experimental myopathy. The groups studied were: (A) control (intact rats that received PEMF sham exposures); (B) rats with myopathy and sacrificed 24 h later; (C) rats with myopathy; (D) rats with myopathy and treated with PEMF; and (E) intact rats treated with PEMF. Groups A, C, D, and E were sacrificed 8 days later. Myopathy was induced by injecting 50 ml of 1% carrageenan l (type IV) once subplantar. Treatment was carried out with PEMF emitting equipment with two flat solenoid disks for 8 consecutive days in groups D and E, at 20 mT and 50 Hz for 30 min/day/rat. The biomarkers were determined by spectrophotometry. The muscles (5/8) were stained with Hematoxylin-Eosin and examined by optic microscopy. Quantitative variables were statistically analyzed by the Fisher test, and categorical applying Pearson?s Chi Squared test at p , 0.05 for all cases. In Groups B and C, the biomarkers were significantly increased compared to A, D, and E groups: fibrinogen (p , 0.001); NO, L-citrulline and carbonyl groups (p , 0.05); SOD (p , 0.01) as well as the percentage of area with inflammatory infiltration (p , 0.001). PEMF caused decreased levels of fibrinogen, L-citrulline, NO, SOD, and carbonyl groups and significant muscle recovery in rats with experimental myopathies.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Vignola, María Belén; Dávila, Soledad; Cremonezzi, David; Simes, Juan Carlos; Palma, José A.; et al.; Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats; Informa Healthcare; Electromagnetic Biology And Medicine; 31; 4; 19-12-2012; 320-332
Compartir
Altmétricas