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dc.contributor.author
Bandeiras, Tiago M.
dc.contributor.author
Refojo, Patricia N.
dc.contributor.author
Todorovic, Smilja
dc.contributor.author
Murgida, Daniel Horacio
dc.contributor.author
Hildebrandt, Peter
dc.contributor.author
Bauer, Christian
dc.contributor.author
Pereira, Manuela M.
dc.contributor.author
Kletzin, Arnulf
dc.contributor.author
Teixeira, Miguel
dc.date.available
2019-09-13T21:55:58Z
dc.date.issued
2009-01
dc.identifier.citation
Bandeiras, Tiago M.; Refojo, Patricia N.; Todorovic, Smilja; Murgida, Daniel Horacio; Hildebrandt, Peter; et al.; The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1787; 1; 1-2009; 37-45
dc.identifier.issn
0005-2728
dc.identifier.uri
http://hdl.handle.net/11336/83606
dc.description.abstract
A novel cytochrome ba complex was isolated from aerobically grown cells of the thermoacidophilic archaeon Acidianus ambivalens. The complex was purified with two subunits, which are encoded by the cbsA and soxN genes. These genes are part of the pentacistronic cbsAB-soxLN-odsN locus. The spectroscopic characterization revealed the presence of three low-spin hemes, two of the b and one of the as-type with reduction potentials of + 200, + 400 and + 160 mV, respectively. The SoxN protein is proposed to harbor the heme b of lower reduction potential and the heme as, and CbsA the other heme b. The soxL gene encodes a Rieske protein, which was expressed in E. coli; its reduction potential was determined to be + 320 mV. Topology predictions showed that SoxN, CbsB and CbsA should contain 12, 9 and one transmembrane α-helices, respectively, with SoxN having a predicted fold very similar to those of the cytochromes b in bc1 complexes. The presence of two quinol binding motifs was also predicted in SoxN. Based on these findings, we propose that the A. ambivalens cytochrome ba complex is analogous to the bc1 complexes of bacteria and mitochondria, however with distinct subunits and heme types.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Archaea
dc.subject
B6f Complex
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Bc1 Complex
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Cytochrome B
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Rieske
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Sulfolobales
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
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Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-03-27T17:54:08Z
dc.journal.volume
1787
dc.journal.number
1
dc.journal.pagination
37-45
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Bandeiras, Tiago M.. Universidade Nova de Lisboa; Portugal
dc.description.fil
Fil: Refojo, Patricia N.. Universidade Nova de Lisboa; Portugal
dc.description.fil
Fil: Todorovic, Smilja. Universidade Nova de Lisboa; Portugal
dc.description.fil
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Technishe Universitat Berlin; Alemania
dc.description.fil
Fil: Hildebrandt, Peter. Technishe Universitat Berlin; Alemania
dc.description.fil
Fil: Bauer, Christian. Darmstadt University of Technology; Alemania
dc.description.fil
Fil: Pereira, Manuela M.. Universidade Nova de Lisboa; Portugal
dc.description.fil
Fil: Kletzin, Arnulf. Darmstadt University of Technology; Alemania
dc.description.fil
Fil: Teixeira, Miguel. Universidade Nova de Lisboa; Portugal
dc.journal.title
Biochimica Et Biophysica Acta-bioenergetics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005272808006865
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.bbabio.2008.09.009
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