Artículo
The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes
Bandeiras, Tiago M.; Refojo, Patricia N.; Todorovic, Smilja; Murgida, Daniel Horacio
; Hildebrandt, Peter; Bauer, Christian; Pereira, Manuela M.; Kletzin, Arnulf; Teixeira, Miguel
Fecha de publicación:
01/2009
Editorial:
Elsevier Science
Revista:
Biochimica Et Biophysica Acta-bioenergetics
ISSN:
0005-2728
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A novel cytochrome ba complex was isolated from aerobically grown cells of the thermoacidophilic archaeon Acidianus ambivalens. The complex was purified with two subunits, which are encoded by the cbsA and soxN genes. These genes are part of the pentacistronic cbsAB-soxLN-odsN locus. The spectroscopic characterization revealed the presence of three low-spin hemes, two of the b and one of the as-type with reduction potentials of + 200, + 400 and + 160 mV, respectively. The SoxN protein is proposed to harbor the heme b of lower reduction potential and the heme as, and CbsA the other heme b. The soxL gene encodes a Rieske protein, which was expressed in E. coli; its reduction potential was determined to be + 320 mV. Topology predictions showed that SoxN, CbsB and CbsA should contain 12, 9 and one transmembrane α-helices, respectively, with SoxN having a predicted fold very similar to those of the cytochromes b in bc1 complexes. The presence of two quinol binding motifs was also predicted in SoxN. Based on these findings, we propose that the A. ambivalens cytochrome ba complex is analogous to the bc1 complexes of bacteria and mitochondria, however with distinct subunits and heme types.
Palabras clave:
Archaea
,
B6f Complex
,
Bc1 Complex
,
Cytochrome B
,
Rieske
,
Sulfolobales
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INQUIMAE)
Articulos de INST.D/QUIM FIS D/L MATERIALES MEDIOAMB Y ENERGIA
Articulos de INST.D/QUIM FIS D/L MATERIALES MEDIOAMB Y ENERGIA
Citación
Bandeiras, Tiago M.; Refojo, Patricia N.; Todorovic, Smilja; Murgida, Daniel Horacio; Hildebrandt, Peter; et al.; The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1787; 1; 1-2009; 37-45
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