Artículo

Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine

Araya, Roberto; Andino Pavlovsky, VictoriaIcon ; Yuste, Rafael; Etchenique, Roberto ArgentinoIcon
Fecha de publicación: 05/2013
Editorial: American Chemical Society
Revista: ACS Chemical Neuroscience
ISSN: 1948-7193
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution.
Palabras clave: Dopamine , Ruthenium , Two Photon , Caged Compound
 
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Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/8018
URL: http://dx.doi.org/10.1021/cn4000692
URL: http://pubs.acs.org/doi/abs/10.1021/cn4000692
Colecciones
Articulos(INQUIMAE)
Articulos de INST.D/QUIM FIS D/L MATERIALES MEDIOAMB Y ENERGIA
Citación
Araya, Roberto; Andino Pavlovsky, Victoria; Yuste, Rafael; Etchenique, Roberto Argentino; Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine; American Chemical Society; ACS Chemical Neuroscience; 4; 8; 5-2013; 1163-1167
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