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dc.contributor.author
Dos Santos Ferreira, Veronica Beatriz
dc.contributor.author
Rocchetta, Iara
dc.contributor.author
Conforti, Visitacion Teresa D.
dc.contributor.author
Bench, Shellie
dc.contributor.author
Feldman, Robert
dc.contributor.author
Levin, Mariano Jorge
dc.date.available
2019-07-19T15:17:06Z
dc.date.issued
2007-03
dc.identifier.citation
Dos Santos Ferreira, Veronica Beatriz; Rocchetta, Iara; Conforti, Visitacion Teresa D.; Bench, Shellie; Feldman, Robert; et al.; Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress; Elsevier Science; Gene; 389; 2; 3-2007; 136-145
dc.identifier.issn
0378-1119
dc.identifier.uri
http://hdl.handle.net/11336/79885
dc.description.abstract
To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e- 10). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cdna Library
dc.subject
Chromium
dc.subject
Est
dc.subject
Microarray
dc.subject
Streptomycin
dc.subject.classification
Inmunología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-18T13:01:38Z
dc.journal.volume
389
dc.journal.number
2
dc.journal.pagination
136-145
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Dos Santos Ferreira, Veronica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Rocchetta, Iara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
dc.description.fil
Fil: Conforti, Visitacion Teresa D.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
dc.description.fil
Fil: Bench, Shellie. University of California; Estados Unidos
dc.description.fil
Fil: Feldman, Robert. University of California; Estados Unidos. SymBio Corporation; Estados Unidos
dc.description.fil
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina. lnstitut Cochin; Francia
dc.journal.title
Gene
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378111906006858
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.gene.2006.10.023


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