Artículo
Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
Dos Santos Ferreira, Veronica Beatriz
; Rocchetta, Iara
; Conforti, Visitacion Teresa D.
; Bench, Shellie; Feldman, Robert; Levin, Mariano Jorge
Fecha de publicación:
03/2007
Editorial:
Elsevier Science
Revista:
Gene
ISSN:
0378-1119
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e- 10). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response.
Palabras clave:
Cdna Library
,
Chromium
,
Est
,
Microarray
,
Streptomycin
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Articulos(INGEBI)
Articulos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Articulos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Citación
Dos Santos Ferreira, Veronica Beatriz; Rocchetta, Iara; Conforti, Visitacion Teresa D.; Bench, Shellie; Feldman, Robert; et al.; Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress; Elsevier Science; Gene; 389; 2; 3-2007; 136-145
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