Mostrar el registro sencillo del ítem
dc.contributor.author
Medina, Lisvaneth
dc.contributor.author
Castillo, Christian
dc.contributor.author
Liempi, Ana
dc.contributor.author
Herbach, Mathias
dc.contributor.author
Cabrera, Gonzalo
dc.contributor.author
Valenzuela, Lucía
dc.contributor.author
Galanti, Norbel
dc.contributor.author
Curto, Maria de Los Angeles
dc.contributor.author
Schijman, Alejandro Gabriel
dc.contributor.author
Kemmerling, Ulrike
dc.date.available
2019-07-18T19:08:59Z
dc.date.issued
2018-10
dc.identifier.citation
Medina, Lisvaneth; Castillo, Christian; Liempi, Ana; Herbach, Mathias; Cabrera, Gonzalo; et al.; Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue; Elsevier Science; Acta Tropica; 186; 10-2018; 35-40
dc.identifier.issn
0001-706X
dc.identifier.uri
http://hdl.handle.net/11336/79831
dc.description.abstract
Congenital Chagas disease, caused by Trypanosoma cruzi (T. cruzi), has become epidemiologically relevant. The probability of congenital transmission depends on the maternal and developing fetal/newborn immune responses, placental factors and importantly, the virulence of the parasite. It has been proposed, that different genotypes of T. cruzi and their associated pathogenicity, virulence and tissue tropism may play an important role in congenital infection. Since there is no laboratory or animal model that recapitulates the complexities of vertical transmission in humans, here we studied parasite infectivity in human placental explants (HPE) as well as in the human trophoblast-derived cell line BeWo of the Y(DTU II) and the VD (TcVI) T. cruzi strains; the latter was isolated from a human case of congenital infection. Our results show that the VD strain is more infective and pathogenic than the Y strain, as demonstrated by qPCR and cell counting as well as by histopathological analysis. The present study constitutes the first approach to study the relationship between parasite two parasite strains from different genotypes and the infection efficiency in human placenta.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Genotypes
dc.subject
Human Placenta
dc.subject
Tropism
dc.subject
Trypanosoma Cruzi
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Differential infectivity of two Trypanosoma cruzi strains in placental cells and tissue
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-17T13:12:56Z
dc.journal.volume
186
dc.journal.pagination
35-40
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Medina, Lisvaneth. Universidad de Chile; Chile
dc.description.fil
Fil: Castillo, Christian. Universidad de Chile; Chile
dc.description.fil
Fil: Liempi, Ana. Universidad de Chile; Chile
dc.description.fil
Fil: Herbach, Mathias. Universidad de Chile; Chile
dc.description.fil
Fil: Cabrera, Gonzalo. Universidad de Chile; Chile
dc.description.fil
Fil: Valenzuela, Lucía. Universidad de Chile; Chile
dc.description.fil
Fil: Galanti, Norbel. Universidad de Chile; Chile
dc.description.fil
Fil: Curto, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Kemmerling, Ulrike. Universidad de Chile; Chile
dc.journal.title
Acta Tropica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.actatropica.2018.07.001
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/30018029
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0001706X18300214
Archivos asociados