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dc.contributor.author
Femia, Lis
dc.contributor.author
Temprana, Carlos Facundo
dc.contributor.author
Santos, Javier
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Carbajal, Maria Laura
dc.contributor.author
Amor, María Silvia
dc.contributor.author
Grasselli, Mariano
dc.contributor.author
Alonso, Silvia del Valle
dc.date.available
2019-07-05T13:22:06Z
dc.date.issued
2012-12
dc.identifier.citation
Femia, Lis; Temprana, Carlos Facundo; Santos, Javier; Carbajal, Maria Laura; Amor, María Silvia; et al.; An Arsenic Fluorescent Compound as a Novel Probe to Study Arsenic-Binding Proteins; Springer; Protein Journal; 31; 8; 12-2012; 656-666
dc.identifier.issn
1572-3887
dc.identifier.uri
http://hdl.handle.net/11336/79194
dc.description.abstract
Arsenic-binding proteins are under continuous research. Their identification and the elucidation of arsenic/ protein interaction mechanisms are important because the biological effects of these complexes may be related not only to arsenic but also to the arsenic/protein structure. Although many proteins bearing a CXXC motif have been found to bind arsenic in vivo, new tools are necessary to identify new arsenic targets and allow research on protein/ arsenic complexes. In this work, we analyzed the performance of the fluorescent compound APAO-FITC (synthesized from p-aminophenylarsenoxide, APAO, and fluorescein isothiocyanate, FITC) in arsenic/protein binding assays using thioredoxin 1 (Trx) as an arsenic-binding protein model. The Trx-APAO-FITC complex was studied through different spectroscopic techniques involving UV-Vis, fluorescence, atomic absorption, infrared and circular dichroism. Our results show that APAO-FITC binds efficiently and specifically to the Trx binding site, labeling the protein fluorescently, without altering its structure and activity. In summary, we were able to study a protein/ arsenic complex model, using APAO-FITC as a labeling probe. The use of APAO-FITC in the identification of different protein and cell targets, as well as in in vivo biodistribution studies, conformational studies of arsenicbinding proteins, and studies for the design of drug delivery systems for arsenic anti-cancer therapies, is highly promising.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Arsenic Carcinogenesis
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Circular Dichroism
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Conformational Studies
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Drug Delivery Systems
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Thioredoxin
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Química Orgánica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
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Métodos de Investigación en Bioquímica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
An Arsenic Fluorescent Compound as a Novel Probe to Study Arsenic-Binding Proteins
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-06-14T18:35:57Z
dc.identifier.eissn
1573-4943
dc.journal.volume
31
dc.journal.number
8
dc.journal.pagination
656-666
dc.journal.pais
Alemania
dc.journal.ciudad
Berlín
dc.description.fil
Fil: Femia, Lis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Temprana, Carlos Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Santos, Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.description.fil
Fil: Carbajal, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Amor, María Silvia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Grasselli, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
dc.description.fil
Fil: Alonso, Silvia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
dc.journal.title
Protein Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10930-012-9441-6
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10930-012-9441-6
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