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Artículo

An Arsenic Fluorescent Compound as a Novel Probe to Study Arsenic-Binding Proteins

Femia, LisIcon ; Temprana, Carlos FacundoIcon ; Santos, JavierIcon ; Carbajal, Maria LauraIcon ; Amor, María Silvia; Grasselli, MarianoIcon ; Alonso, Silvia del ValleIcon
Fecha de publicación: 12/2012
Editorial: Springer
Revista: Protein Journal
ISSN: 1572-3887
e-ISSN: 1573-4943
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Química Orgánica; Métodos de Investigación en Bioquímica

Resumen

Arsenic-binding proteins are under continuous research. Their identification and the elucidation of arsenic/ protein interaction mechanisms are important because the biological effects of these complexes may be related not only to arsenic but also to the arsenic/protein structure. Although many proteins bearing a CXXC motif have been found to bind arsenic in vivo, new tools are necessary to identify new arsenic targets and allow research on protein/ arsenic complexes. In this work, we analyzed the performance of the fluorescent compound APAO-FITC (synthesized from p-aminophenylarsenoxide, APAO, and fluorescein isothiocyanate, FITC) in arsenic/protein binding assays using thioredoxin 1 (Trx) as an arsenic-binding protein model. The Trx-APAO-FITC complex was studied through different spectroscopic techniques involving UV-Vis, fluorescence, atomic absorption, infrared and circular dichroism. Our results show that APAO-FITC binds efficiently and specifically to the Trx binding site, labeling the protein fluorescently, without altering its structure and activity. In summary, we were able to study a protein/ arsenic complex model, using APAO-FITC as a labeling probe. The use of APAO-FITC in the identification of different protein and cell targets, as well as in in vivo biodistribution studies, conformational studies of arsenicbinding proteins, and studies for the design of drug delivery systems for arsenic anti-cancer therapies, is highly promising.
Palabras clave: Arsenic Carcinogenesis , Circular Dichroism , Conformational Studies , Drug Delivery Systems , Thioredoxin
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/79194
URL: http://link.springer.com/article/10.1007%2Fs10930-012-9441-6
DOI: http://dx.doi.org/10.1007/s10930-012-9441-6
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(IMBICE)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA CELULAR (I)
Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Femia, Lis; Temprana, Carlos Facundo; Santos, Javier; Carbajal, Maria Laura; Amor, María Silvia; et al.; An Arsenic Fluorescent Compound as a Novel Probe to Study Arsenic-Binding Proteins; Springer; Protein Journal; 31; 8; 12-2012; 656-666
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