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dc.contributor.author
Manucha, Walter Ariel Fernando
dc.contributor.author
Garramuño, Patricia
dc.date.available
2019-07-04T21:06:41Z
dc.date.issued
2008-12
dc.identifier.citation
Manucha, Walter Ariel Fernando; Garramuño, Patricia; Cytoprotective role of nitric oxide associated with Hsp70 expression in neonatal obstructive nephropathy; Academic Press Inc Elsevier Science; Nitric Oxide-Biology and Chemistry; 18; 3; 12-2008; 204-215
dc.identifier.issn
1089-8603
dc.identifier.uri
http://hdl.handle.net/11336/79179
dc.description.abstract
Nitric oxide (NO) has emerged as an important endogenous inhibitor of apoptosis. In this study, we postulated that the mechanism of apoptosis inhibition by NO would include stimulation of heat shock protein 70 (Hsp70) expression. Rats were subjected to unilateral ureteral obstruction (UUO) or sham operation, and kidneys were harvested 5 and 14 days after obstruction. After 14 days of obstruction, decreased endogenous NO and lower inducible nitric oxide synthase (iNOS) expression at mRNA and protein levels associated with downregulation of Hsp70 protein expression were shown in apoptosis induction, regulated by mitochondrial signal pathway, through the increased pro-apoptotic ratio Bax/BcL2 and consequently caspase 3 activity. Conversely, 5 days after kidney obstruction, increased Hsp70 expression linked to increase NO and iNOS expression at transcriptional and post-transcriptional levels with absence of apoptotic response, were demonstrated. In obstructed neonatal rats, in vivo administration of l-Arginine induced heat shock protein 70 (Hsp70) expression, which was associated with cytoprotection from apoptosis and transiently decreased nicotinamide adenine dinucleotide phosphate reduced form (NADPH) oxidase activity. Opposite effects were obtained after nitro l-Arginine methyl ester (l-NAME) treatment. The interaction between B-cell lymphoma 2 anti-apoptotic members (BcL2) and Hsp70 in the presence of l-Arginine and l-NAME, was determined by coimmunoprecipitation. Binding of BcL2 and Hsp70 increased after l-Arginine administration. These findings suggest that NO can produce resistance to obstruction-induced cell death by mitochondrial apoptotic pathway, through the induction of Hsp70 expression, in neonatal unilateral ureteral obstruction.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Apoptosis
dc.subject
Bcl2
dc.subject
Caspase 3
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Hsp70
dc.subject
Neonatal Unilateral Ureteral Obstruction
dc.subject
Nitric Oxide
dc.subject.classification
Medicina Critica y de Emergencia
dc.subject.classification
Medicina Clínica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Cytoprotective role of nitric oxide associated with Hsp70 expression in neonatal obstructive nephropathy
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-06-10T21:25:03Z
dc.journal.volume
18
dc.journal.number
3
dc.journal.pagination
204-215
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
dc.description.fil
Fil: Garramuño, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
dc.journal.title
Nitric Oxide-Biology and Chemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.niox.2008.01.005
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1089860308000049
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