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Artículo

Cytoprotective role of nitric oxide associated with Hsp70 expression in neonatal obstructive nephropathy

Manucha, Walter Ariel FernandoIcon ; Garramuño, PatriciaIcon
Fecha de publicación: 12/2008
Editorial: Academic Press Inc Elsevier Science
Revista: Nitric Oxide-Biology and Chemistry
ISSN: 1089-8603
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Medicina Critica y de Emergencia

Resumen

Nitric oxide (NO) has emerged as an important endogenous inhibitor of apoptosis. In this study, we postulated that the mechanism of apoptosis inhibition by NO would include stimulation of heat shock protein 70 (Hsp70) expression. Rats were subjected to unilateral ureteral obstruction (UUO) or sham operation, and kidneys were harvested 5 and 14 days after obstruction. After 14 days of obstruction, decreased endogenous NO and lower inducible nitric oxide synthase (iNOS) expression at mRNA and protein levels associated with downregulation of Hsp70 protein expression were shown in apoptosis induction, regulated by mitochondrial signal pathway, through the increased pro-apoptotic ratio Bax/BcL2 and consequently caspase 3 activity. Conversely, 5 days after kidney obstruction, increased Hsp70 expression linked to increase NO and iNOS expression at transcriptional and post-transcriptional levels with absence of apoptotic response, were demonstrated. In obstructed neonatal rats, in vivo administration of l-Arginine induced heat shock protein 70 (Hsp70) expression, which was associated with cytoprotection from apoptosis and transiently decreased nicotinamide adenine dinucleotide phosphate reduced form (NADPH) oxidase activity. Opposite effects were obtained after nitro l-Arginine methyl ester (l-NAME) treatment. The interaction between B-cell lymphoma 2 anti-apoptotic members (BcL2) and Hsp70 in the presence of l-Arginine and l-NAME, was determined by coimmunoprecipitation. Binding of BcL2 and Hsp70 increased after l-Arginine administration. These findings suggest that NO can produce resistance to obstruction-induced cell death by mitochondrial apoptotic pathway, through the induction of Hsp70 expression, in neonatal unilateral ureteral obstruction.
Palabras clave: Apoptosis , Bcl2 , Caspase 3 , Hsp70 , Neonatal Unilateral Ureteral Obstruction , Nitric Oxide
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/79179
DOI: http://dx.doi.org/10.1016/j.niox.2008.01.005
URL: https://www.sciencedirect.com/science/article/pii/S1089860308000049
Colecciones
Articulos(IMBECU)
Articulos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Citación
Manucha, Walter Ariel Fernando; Garramuño, Patricia; Cytoprotective role of nitric oxide associated with Hsp70 expression in neonatal obstructive nephropathy; Academic Press Inc Elsevier Science; Nitric Oxide-Biology and Chemistry; 18; 3; 12-2008; 204-215
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