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dc.contributor.author Cardozo Gizzi, Andres Mauricio
dc.contributor.author Prucca, Cesar German
dc.contributor.author Gaveglio, Virginia Lucía
dc.contributor.author Renner, Marianne L.
dc.contributor.author Pasquaré, Susana J.
dc.contributor.author Caputto, Beatriz Leonor
dc.date.available 2016-09-09T20:59:50Z
dc.date.issued 2015-10
dc.identifier.citation Cardozo Gizzi, Andres Mauricio; Prucca, Cesar German; Gaveglio, Virginia Lucía; Renner, Marianne L.; Pasquaré, Susana J.; et al.; The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos; American Society For Biochemistry And Molecular Biology; Journal Of Biological Chemistry; 290; 10-2015; 29578-29592
dc.identifier.issn 0021-9258
dc.identifier.uri http://hdl.handle.net/11336/7601
dc.description.abstract Phosphatidic acid (PA) is a central precursor for membrane phospholipid biosynthesis. Lipin family is a Mg-dependent type I PA phosphatase,involved in de novo synthesis of neutral lipids and of phospholipids. The regulation of Lipin activity may govern the pathways by which these lipids aresynthesized and control the cellular levels ofimportant signaling lipids. On the other hand, the proto-oncoprotein c-Fos has an emerging role in glycerolipid synthesis regulation: by interacting with key synthetizing enzymes it is able toincrease overall phopho- and glyco- lipid synthesis.We studied the Lipin 1β enzyme activity in a cell-free system using PA/Triton X-100 mixed micelles as substrate, analyzing it in the presence/absence of c-Fos. We found that Lipin 1β kcat increases around 40% in the presence of c- Fos, with no change in the Lipin 1β affinity for the PA/Triton X-100 mixed micelles. We also probed a physical interaction between both proteins. While the c-Fos domain involved in Lipin activation is its basic domain (BD), the interaction domain is mapped to the c-Fos N-terminal. In conclusion, we provide evidence for a novel positive regulator of Lipin 1β PA phosphatase activity that is not achieved via altering its subcellular localization or affinity for membranesbut rather through directly increasing its catalytic efficiency.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Society For Biochemistry And Molecular Biology
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject LIPIN
dc.subject C-FOS
dc.subject PHOSPHATIDIC ACID
dc.subject PHOSPHATIDIC ACID PHOSPHATASE
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2016-05-10T14:31:31Z
dc.journal.volume 290
dc.journal.pagination 29578-29592
dc.journal.pais Estados Unidos
dc.journal.ciudad Bethesda
dc.description.fil Fil: Cardozo Gizzi, Andres Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.description.fil Fil: Prucca, Cesar German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.description.fil Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
dc.description.fil Fil: Renner, Marianne L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (p); Argentina
dc.description.fil Fil: Pasquaré, Susana J.. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
dc.description.fil Fil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
dc.journal.title Journal Of Biological Chemistry
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/290/49/29578
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1074/jbc.M115.678821


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)