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Artículo

The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos

Cardozo Gizzi, Andres MauricioIcon ; Prucca, Cesar GermanIcon ; Gaveglio, Virginia LucíaIcon ; Renner, Marianne L.; Pasquaré, Susana J.; Caputto, Beatriz LeonorIcon
Fecha de publicación: 10/2015
Editorial: American Society For Biochemistry And Molecular Biology
Revista: Journal Of Biological Chemistry
ISSN: 0021-9258
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Phosphatidic acid (PA) is a central precursor for membrane phospholipid biosynthesis. Lipin family is a Mg-dependent type I PA phosphatase,involved in de novo synthesis of neutral lipids and of phospholipids. The regulation of Lipin activity may govern the pathways by which these lipids aresynthesized and control the cellular levels ofimportant signaling lipids. On the other hand, the proto-oncoprotein c-Fos has an emerging role in glycerolipid synthesis regulation: by interacting with key synthetizing enzymes it is able toincrease overall phopho- and glyco- lipid synthesis.We studied the Lipin 1β enzyme activity in a cell-free system using PA/Triton X-100 mixed micelles as substrate, analyzing it in the presence/absence of c-Fos. We found that Lipin 1β kcat increases around 40% in the presence of c- Fos, with no change in the Lipin 1β affinity for the PA/Triton X-100 mixed micelles. We also probed a physical interaction between both proteins. While the c-Fos domain involved in Lipin activation is its basic domain (BD), the interaction domain is mapped to the c-Fos N-terminal. In conclusion, we provide evidence for a novel positive regulator of Lipin 1β PA phosphatase activity that is not achieved via altering its subcellular localization or affinity for membranesbut rather through directly increasing its catalytic efficiency.
Palabras clave: Lipin , C-Fos , Phosphatidic Acid , Phosphatidic Acid Phosphatase
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/7601
URL: http://www.jbc.org/content/290/49/29578
DOI: http://dx.doi.org/10.1074/jbc.M115.678821
Colecciones
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos(INIBIBB)
Articulos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Citación
Cardozo Gizzi, Andres Mauricio; Prucca, Cesar German; Gaveglio, Virginia Lucía; Renner, Marianne L.; Pasquaré, Susana J.; et al.; The catalytic efficiency of Lipin 1beta increases by physically interacting with the protooncoprotein c-Fos; American Society For Biochemistry And Molecular Biology; Journal Of Biological Chemistry; 290; 10-2015; 29578-29592
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