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dc.contributor.author
Alvarez, Lucía Paula  
dc.contributor.author
Marcellino, R. B.  
dc.contributor.author
Martinez, A.  
dc.contributor.author
Robles, C. A.  
dc.date.available
2019-04-15T15:42:41Z  
dc.date.issued
2016-11-06  
dc.identifier.citation
Alvarez, Lucía Paula; Marcellino, R. B.; Martinez, A.; Robles, C. A.; Duplex PCR for the diagnosis of Brucella melitensis and its differentiation from the REV-1 vaccine strain; Elsevier Science; Journal of Small Ruminant Research; 146; 6-11-2016; 1-4  
dc.identifier.issn
0921-4488  
dc.identifier.uri
http://hdl.handle.net/11336/74375  
dc.description.abstract
Caprine brucellosis is a chronic infectious disease caused by Brucella melitensis, whose main symptoms are abortion in females and orchitis in males. Vaccination by mistake of pregnant goats with the REV-1 vaccine may cause abortions. The aim of this study was to develop a duplex PCR that could identify and differentiate the DNA of B. melitensis field strains from that of the REV-1 vaccine strain. The results showed that the duplex PCR performed on DNA isolated from infected tissues is very convenient as it significantly shortens the time of detection of the etiological agent, yields a reliable result and has an acceptable cost.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Goats  
dc.subject
Brucella Melitensis  
dc.subject
Rev-1  
dc.subject
Molecular Diagnosis  
dc.subject
Pcr  
dc.subject.classification
Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Duplex PCR for the diagnosis of Brucella melitensis and its differentiation from the REV-1 vaccine strain  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-04-11T19:49:37Z  
dc.journal.volume
146  
dc.journal.pagination
1-4  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Alvarez, Lucía Paula. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina  
dc.description.fil
Fil: Marcellino, R. B.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina  
dc.description.fil
Fil: Martinez, A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina  
dc.description.fil
Fil: Robles, C. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina  
dc.journal.title
Journal of Small Ruminant Research  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.smallrumres.2016.11.004  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0921448816303133?via%3Dihub  

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