Artículo

Duplex PCR for the diagnosis of Brucella melitensis and its differentiation from the REV-1 vaccine strain

Alvarez, Lucía PaulaIcon ; Marcellino, R. B.; Martinez, A.; Robles, C. A.
Fecha de publicación: 06/11/2016
Editorial: Elsevier Science
Revista: Journal of Small Ruminant Research
ISSN: 0921-4488
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

Caprine brucellosis is a chronic infectious disease caused by Brucella melitensis, whose main symptoms are abortion in females and orchitis in males. Vaccination by mistake of pregnant goats with the REV-1 vaccine may cause abortions. The aim of this study was to develop a duplex PCR that could identify and differentiate the DNA of B. melitensis field strains from that of the REV-1 vaccine strain. The results showed that the duplex PCR performed on DNA isolated from infected tissues is very convenient as it significantly shortens the time of detection of the etiological agent, yields a reliable result and has an acceptable cost.
Palabras clave: Goats , Brucella Melitensis , Rev-1 , Molecular Diagnosis , Pcr
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/74375
DOI: http://dx.doi.org/10.1016/j.smallrumres.2016.11.004
URL: https://www.sciencedirect.com/science/article/pii/S0921448816303133?via%3Dihub
Colecciones
Articulos(CCT - PATAGONIA NORTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - PATAGONIA NORTE
Citación
Alvarez, Lucía Paula; Marcellino, R. B.; Martinez, A.; Robles, C. A.; Duplex PCR for the diagnosis of Brucella melitensis and its differentiation from the REV-1 vaccine strain; Elsevier Science; Journal of Small Ruminant Research; 146; 6-11-2016; 1-4
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