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dc.contributor.author
Krüger, Alejandra
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dc.contributor.author
Lucchesi, Paula Maria Alejandra
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dc.contributor.author
Parma, Alberto Ernesto
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dc.date.available
2019-03-13T17:31:11Z
dc.date.issued
2011-01
dc.identifier.citation
Krüger, Alejandra; Lucchesi, Paula Maria Alejandra; Parma, Alberto Ernesto; Verotoxins in bovine and meat verotoxin-producing Escherichia coli isolates: Type, number of variants, and relationship to cytotoxicity; American Society for Microbiology; Applied And Environmental Microbiology; 77; 1; 1-2011; 73-79
dc.identifier.issn
0099-2240
dc.identifier.uri
http://hdl.handle.net/11336/71492
dc.description.abstract
In this study, we determined vt subtypes and evaluated verotoxicity in basal as well as induced conditions of verotoxin-producing Escherichia coli (VTEC) strains isolated from cattle and meat products. Most (87%) of the 186 isolates carried a vt2 gene. Moreover, the vt2 subtype, which is associated with serious disease, was present in 42% of our VTEC collection. The other vt subtypes detected were vt1, vt1d, vt2vha, vt2vhb, vt2O118, vt2d (mucus activatable), and vt2g. A total of 41 (22%) of the isolates possessed more than one vt subtype in its genome, and among them the most frequent combination was vt1/vt2, but we also observed multiple combinations among vt2 subtypes. Differences in verotoxicity titers were found among a selection of 54 isolates. Among isolates with a single vt2 variant, those carrying the vt2 subtype had high titers under both uninduced and induced conditions. However, the highest increase in cytotoxicity under mitomycin C treatment was detected among the strains carrying vt2vha or vt2hb variants. Notably, the isolates carrying the vt1 subtype showed a lesser increase than that of most of the vt2-positive VTEC strains. Furthermore, the presence of more than one vt gene variant in the same isolate was not reflected in higher titers, and generally the titers were lower than those for strains with only one gene variant. The main observation was that both basal and induced cytotoxic effects seemed to be associated with the type and number of vt variants more than with the serotype or origin of the isolate.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Verotoxins
dc.subject
Cytotoxicity
dc.subject
Bovine
dc.subject
Meat
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Verotoxins in bovine and meat verotoxin-producing Escherichia coli isolates: Type, number of variants, and relationship to cytotoxicity
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-23T18:54:28Z
dc.journal.volume
77
dc.journal.number
1
dc.journal.pagination
73-79
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Krüger, Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Lucchesi, Paula Maria Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Parma, Alberto Ernesto. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Applied And Environmental Microbiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/AEM.01445-10
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/77/1/73
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