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Artículo

Verotoxins in bovine and meat verotoxin-producing Escherichia coli isolates: Type, number of variants, and relationship to cytotoxicity

Krüger, AlejandraIcon ; Lucchesi, Paula Maria AlejandraIcon ; Parma, Alberto ErnestoIcon
Fecha de publicación: 01/2011
Editorial: American Society for Microbiology
Revista: Applied And Environmental Microbiology
ISSN: 0099-2240
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

In this study, we determined vt subtypes and evaluated verotoxicity in basal as well as induced conditions of verotoxin-producing Escherichia coli (VTEC) strains isolated from cattle and meat products. Most (87%) of the 186 isolates carried a vt2 gene. Moreover, the vt2 subtype, which is associated with serious disease, was present in 42% of our VTEC collection. The other vt subtypes detected were vt1, vt1d, vt2vha, vt2vhb, vt2O118, vt2d (mucus activatable), and vt2g. A total of 41 (22%) of the isolates possessed more than one vt subtype in its genome, and among them the most frequent combination was vt1/vt2, but we also observed multiple combinations among vt2 subtypes. Differences in verotoxicity titers were found among a selection of 54 isolates. Among isolates with a single vt2 variant, those carrying the vt2 subtype had high titers under both uninduced and induced conditions. However, the highest increase in cytotoxicity under mitomycin C treatment was detected among the strains carrying vt2vha or vt2hb variants. Notably, the isolates carrying the vt1 subtype showed a lesser increase than that of most of the vt2-positive VTEC strains. Furthermore, the presence of more than one vt gene variant in the same isolate was not reflected in higher titers, and generally the titers were lower than those for strains with only one gene variant. The main observation was that both basal and induced cytotoxic effects seemed to be associated with the type and number of vt variants more than with the serotype or origin of the isolate.
Palabras clave: Verotoxins , Cytotoxicity , Bovine , Meat
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/71492
DOI: http://dx.doi.org/10.1128/AEM.01445-10
URL: https://aem.asm.org/content/77/1/73
Colecciones
Articulos(CIVETAN)
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Citación
Krüger, Alejandra; Lucchesi, Paula Maria Alejandra; Parma, Alberto Ernesto; Verotoxins in bovine and meat verotoxin-producing Escherichia coli isolates: Type, number of variants, and relationship to cytotoxicity; American Society for Microbiology; Applied And Environmental Microbiology; 77; 1; 1-2011; 73-79
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