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dc.contributor.author
Rodriguez, Ana Virginia
dc.contributor.author
Baigori, Mario Domingo
dc.contributor.author
Alvarez, Gladis Susana
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Castro, Guillermo Raul
dc.contributor.author
Oliver, Guillermo
dc.date.available
2019-02-18T19:54:50Z
dc.date.issued
2001-10
dc.identifier.citation
Rodriguez, Ana Virginia; Baigori, Mario Domingo; Alvarez, Gladis Susana; Castro, Guillermo Raul; Oliver, Guillermo; Phosphatidylinositol-specific phospholipase C activity in Lactobacillus rhamnosus with capacity to translocate; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 204; 1; 10-2001; 33-38
dc.identifier.issn
0378-1097
dc.identifier.uri
http://hdl.handle.net/11336/70395
dc.description.abstract
Phosphatidylinositol-specific phospholipase C (PI-PLC) activity was investigated in 25 different lactic acid bacteria (LAB) strains belonging to the genera Lactobacillus, Weisella, and Enterococcus. PI-PLC activity was detected in 44% of the strains studied in culture medium without carbon source. From the PI-PLC positive strains, Lactobacillus rhamnosus ATCC 7469 was selected for translocation studies. Healthy mice were orally administered with a daily dose of 2.0U109 of viable L. rhamnosus suspension. Viable bacteria were detected in liver and spleen of mice fed with LAB for 7 days. Bacterial colonies isolated from liver were biochemically characterized, and further subjected to randomly amplified polymorphic DNA. Amplification patterns of five strains displayed identical profiles to L. rhamnosus. PI-PLC activity was determined in the strains recovered from liver.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Phospholipasa
dc.subject
Translocation
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Probiotic
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Lactobacillus
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Phosphatidylinositol-specific phospholipase C activity in Lactobacillus rhamnosus with capacity to translocate
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-02-12T14:20:18Z
dc.journal.volume
204
dc.journal.number
1
dc.journal.pagination
33-38
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Rodriguez, Ana Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Oliver, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.journal.title
FEMS Microbiology Letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1111/j.1574-6968.2001.tb10858.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1574-6968.2001.tb10858.x?sid=nlm%3Apubmed
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