Phosphatidylinositol-specific phospholipase C activity in Lactobacillus rhamnosus with capacity to translocate

Phosphatidylinositol-specific phospholipase C (PI-PLC) activity was investigated in 25 different lactic acid bacteria (LAB) strains belonging to the genera Lactobacillus, Weisella, and Enterococcus. PI-PLC activity was detected in 44% of the strains studied in culture medium without carbon source. From the PI-PLC positive strains, Lactobacillus rhamnosus ATCC 7469 was selected for translocation studies. Healthy mice were orally administered with a daily dose of 2.0U109 of viable L. rhamnosus suspension. Viable bacteria were detected in liver and spleen of mice fed with LAB for 7 days. Bacterial colonies isolated from liver were biochemically characterized, and further subjected to randomly amplified polymorphic DNA. Amplification patterns of five strains displayed identical profiles to L. rhamnosus. PI-PLC activity was determined in the strains recovered from liver.

Palabras clave: Phospholipasa , Translocation , Probiotic , Lactobacillus

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

Identificadores

URI: http://hdl.handle.net/11336/70395

DOI: https://doi.org/10.1111/j.1574-6968.2001.tb10858.x

URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1574-6968.2001.tb10858.x?sid=n

Colecciones

Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)

Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)

Citación

Rodriguez, Ana Virginia; Baigori, Mario Domingo; Alvarez, Gladis Susana; Castro, Guillermo Raul; Oliver, Guillermo; Phosphatidylinositol-specific phospholipase C activity in Lactobacillus rhamnosus with capacity to translocate; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 204; 1; 10-2001; 33-38

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