Artículo
Lysine transporters in human trypanosomatid pathogens
Inbar, Ehud; Canepa, Gaspar Exequiel
; Carrillo, Carolina
; Glaser, Fabian; Grotemeyer, Marianne Suter; Rentsch, Doris; Zilberstein, Dan; Pereira, Claudio Alejandro
Fecha de publicación:
01/2012
Editorial:
Springer Wien
Revista:
Amino Acids
ISSN:
0939-4451
e-ISSN:
1438-2199
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In previous studies we characterized arginine transporter genes from Trypanosoma cruzi and Leishmania donovani, the etiological agents of chagas disease and kala azar, respectively, both fatal diseases in humans. Unlike arginine transporters in higher eukaryotes that transport also lysine, these parasite transporters translocate only arginine. This phenomenon prompted us to identify and characterize parasite lysine transporters. Here we demonstrate that LdAAP7 and TcAAP7 encode lysine-specific permeases in L. donovani and T. cruzi, respectively. These two lysine permeases are both members of the large amino acid/auxin permease family and share certain biochemical properties, such as specificity and Km. However, we evidence that LdAAP7 and TcAAP7 differ in their regulation and localization, such differences are likely a reflection of the dissimilar L. donovani and T. cruzi life cycles. Failed attempts to delete both alleles of LdAAP7 support the premise that this is an essential gene that encodes the only lysine permeases expressed in L. donovani promastigotes and T. cruzi epimastigotes, respectively.
Palabras clave:
Amino Acid Transport
,
Leishmania
,
Lysine Transport
,
Trypanosoma
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Articulos(ICT - MILSTEIN)
Articulos de INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN"
Articulos de INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN"
Articulos(IDIM)
Articulos de INST.DE INVEST.MEDICAS
Articulos de INST.DE INVEST.MEDICAS
Citación
Inbar, Ehud; Canepa, Gaspar Exequiel; Carrillo, Carolina; Glaser, Fabian; Grotemeyer, Marianne Suter; et al.; Lysine transporters in human trypanosomatid pathogens; Springer Wien; Amino Acids; 42; 1; 1-2012; 347-360
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