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dc.contributor.author
Wurm, Christian A.
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Kolmakov, Kirill
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Göttfert, Fabian
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Ta, Haisen
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Bossi, Mariano Luis
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Schill, Heiko
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Berning, Sebastian
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Jakobs, Stefan
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Donnert, Gerald
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Belov, Vladimir N.
dc.contributor.author
Hell, Stefan W.
dc.date.available
2019-01-08T21:07:12Z
dc.date.issued
2012-09
dc.identifier.citation
Wurm, Christian A.; Kolmakov, Kirill; Göttfert, Fabian; Ta, Haisen; Bossi, Mariano Luis; et al.; Novel red fluorophores with superior performance in STED microscopy; Springer Open; Optical Nanoscopy; 1; 1; 9-2012; 1-7
dc.identifier.issn
2192-2853
dc.identifier.uri
http://hdl.handle.net/11336/67724
dc.description.abstract
In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al.
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application/pdf
dc.language.iso
eng
dc.publisher
Springer Open
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Bioconjugation
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Confocal
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Fluorescence
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Microscopy
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Nanoscopy
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Rhodamines
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Sted
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Stimulated Emission Depletion
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Super-Resolution
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Otras Ciencias Químicas
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Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Novel red fluorophores with superior performance in STED microscopy
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
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info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-02T19:09:26Z
dc.journal.volume
1
dc.journal.number
1
dc.journal.pagination
1-7
dc.journal.pais
Alemania
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dc.description.fil
Fil: Wurm, Christian A.. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; Alemania
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Fil: Kolmakov, Kirill. Max Planck Institute for Biophysical Chemistry; Alemania
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Fil: Göttfert, Fabian. Max Planck Institute for Biophysical Chemistry; Alemania
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Fil: Ta, Haisen. Max Planck Institute for Biophysical Chemistry; Alemania
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Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
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Fil: Schill, Heiko. Max Planck Institute for Biophysical Chemistry; Alemania
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Fil: Berning, Sebastian. Max Planck Institute for Biophysical Chemistry; Alemania
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Fil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; Alemania
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Fil: Donnert, Gerald. Max Planck Institute for Biophysical Chemistry; Alemania
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Fil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania
dc.journal.title
Optical Nanoscopy
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1186/2192-2853-1-7
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1186/2192-2853-1-7
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