Artículo
Novel red fluorophores with superior performance in STED microscopy
Wurm, Christian A.; Kolmakov, Kirill; Göttfert, Fabian; Ta, Haisen; Bossi, Mariano Luis
; Schill, Heiko; Berning, Sebastian; Jakobs, Stefan; Donnert, Gerald; Belov, Vladimir N.; Hell, Stefan W.
Fecha de publicación:
09/2012
Editorial:
Springer Open
Revista:
Optical Nanoscopy
ISSN:
2192-2853
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al.
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Articulos(INQUIMAE)
Articulos de INST.D/QUIM FIS D/L MATERIALES MEDIOAMB Y ENERGIA
Articulos de INST.D/QUIM FIS D/L MATERIALES MEDIOAMB Y ENERGIA
Citación
Wurm, Christian A.; Kolmakov, Kirill; Göttfert, Fabian; Ta, Haisen; Bossi, Mariano Luis; et al.; Novel red fluorophores with superior performance in STED microscopy; Springer Open; Optical Nanoscopy; 1; 1; 9-2012; 1-7
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