Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

The response to postnatal stress: Amino acids transporters and pkc activity

Odeon, Maria MercedesIcon ; Salatino, Adrián EmanuelIcon ; Rodríguez, Carla Beatriz; Scolari, Mariano José; Acosta, Gabriela BeatrizIcon
Fecha de publicación: 07/2010
Editorial: Springer/Plenum Publishers
Revista: Neurochemical Research
ISSN: 0364-3190
e-ISSN: 1573-6903
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

During postnatal development, the central nervous system (CNS) is highly sensitive to effects of drugs, stressors and environment. In this study, we evaluated the effect of acute cold stress on g-aminobutyric acid (GABA) and L-serine (L-Ser) transporters in vitro, using the uptake of [3H]GABA and [3H]L-Ser by synaptosomes-enriched fractions isolated from rat cerebral cortex during postnatal development. We found that the maximal velocity (Vmax) L-Ser uptake after stress session was increased in almost all ages studied, except at postnatal-days (PD) 5, whereas the values of Km (uptake affinity) decreased in all ages from 5 to 21 PD compared with the control group. The highest values of Vmax and Km were obtained at PD7. At the same time, in GABA uptake, the Km decreased although Vmax increased in all ages considered after cold stress during development. Finally, we investigated the mechanism by which cells regulate the substrate affinity of L-serine and GABA transporters. We demonstrated a significantly increase in PKC activity to PD5 from PD21. Pretreatment with PKC inhibitor: staurosporine (SP) led to a restoration of control uptake in several postnatal-days suggesting a relationship between amino acids uptake and PKC activation. These findings suggest that a single exposure to postnatal cold stress at different periods after birth modifies both GABA and L-Ser transporters and the related increase in PKC activity could be intracellular events that participate in neuronal plasticity by early-life stress, which could be relevant to function of transporters in the adult rat brain.
Palabras clave: Acute Cold Stress , Gaba , L-Serina , Pkc
Ver el registro completo
 
Archivos asociados
Tamaño: 565.3Kb
Formato: PDF
.
Solicitar
Licencia
info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/67668
DOI: https://doi.org/10.1007/s11064-010-0153-z
URL: https://link.springer.com/article/10.1007/s11064-010-0153-z
Colecciones
Articulos(ININFA)
Articulos de INST.DE INVEST.FARMACOLOGICAS (I)
Citación
Odeon, Maria Mercedes; Salatino, Adrián Emanuel; Rodríguez, Carla Beatriz; Scolari, Mariano José; Acosta, Gabriela Beatriz; The response to postnatal stress: Amino acids transporters and pkc activity; Springer/Plenum Publishers; Neurochemical Research; 35; 7; 7-2010; 967-975
Compartir
Altmétricas
 

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • X Conicet Digital
  • Instagram
  • YouTube
  • Sound Cloud
  • LinkedIn

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES