Artículo
Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes
Araujo, A. S. R.; Seibel, F. E. R.; Fernandes, T.; Llesuy, Susana Francisca
; Kucharski, L.; Belló Klein, A.
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
07/2011
Editorial:
John Wiley & Sons Ltd
Revista:
Cell Biochemistry And Function
ISSN:
0263-6484
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Thyroid hormones modulate haemoglobin and reactive oxygen species (ROS) production, leading to antioxidant changes. This study evaluated the antioxidant response to ROS in erythrocytes in hypothyroid and hyperthyroid rats. Wistar rats were divided into four groups: control; hyperthyroid (T4-12mgl-1 in drinking water); sham operated (simulation of thyroidectomy); and hypothyroid (thyroidectomized). Four weeks after, blood was collected and haemoglobin and T4 levels, lipid peroxidation (LPO), protein oxidation, superoxide dismutase (SOD), catalase (CAT) , glutathione S-transferase (GST) and glutathione peroxidase (GPx) activities, and total radical antioxidant potential (TRAP) were measured. SOD, CAT and GST immunocontent was evaluated. Haemoglobin levels were increased in hyperthyroid erythrocytes. LPO and carbonyls were augmented (65% and 55%, respectively) in hyperthyroid and reduced (31% and 56%, respectively) in hypothyroid group. SOD and CAT activities have not changed, as well as CAT immunocontent. TRAP was diminished in both hyperthyroid and hypothyroid groups (36% and 37%, respectively). GST activity and immunocontent, as well as GPx activity, were increased in hyper and hypothyroid rats. The data suggest that thyroid hormone changes determine ROS concentration changes and decrease of some antioxidant defences that would lead to a compensatory answer of the GST and GPx enzymes, which could be consider as credible biomarkers.
Palabras clave:
Blood-Biomarkers
,
Gpx
,
Gst
,
Hyperthyroidism
,
Hypothyroidism
,
Oxidative Damage
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Identificadores
Colecciones
Articulos(IBIMOL)
Articulos de INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR
Articulos de INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR
Citación
Araujo, A. S. R.; Seibel, F. E. R.; Fernandes, T.; Llesuy, Susana Francisca; Kucharski, L.; et al.; Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes; John Wiley & Sons Ltd; Cell Biochemistry And Function; 29; 5; 7-2011; 408-413
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