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Artículo

Control of von Willebrand factor multimer size by a fibronectin-related substance

Kempfer, Ana CatalinaIcon ; Farias, Cristina ElenaIcon ; Amaral, María MartaIcon ; Silaf, María R.; Woods, Adriana InésIcon ; Carballo, Gonzalo A.; Lazzari, María ÁngelaIcon
Fecha de publicación: 07/2003
Editorial: Lippincott Williams
Revista: Blood Coagulation & Fibrinolysis : An International Journal In Haemostasis And Thrombosis.
ISSN: 0957-5235
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Medicina Básica

Resumen

Fraction (F) II and FIII obtained by heparin-Sepharose after digestion of partially purified fibronectin (FN) with cathepsin D and F3, obtained like FIII but from untreated FN, exerted activity (arFN) on unfolded purified von Willebrand factor (vWF) that controls vWF multimer size. Our aim was to evaluate the arFN of F from commercial FN, commercial 30 kDa (with heparin affinity), 45 kDa (gelatin affinity) and 70 kDa FN fragments (gelatin and heparin affinity) and whole FN. The arFN was detected in FII, FIII, F2, F3, 30 kDa, 45 kDa and 70 kDa fragments. The least contaminated sample was the 30 kDa commercial fragment. Characterization studies of this sample revealed two bands: a blurred band of approximately 60 kDa and a sharp major band of 32 ± 6 kDa. The 32 ± 6 kDa band fragment failed to produce arFN because it was stronger than in F2 and FIII band fragments at the same position and with the same arFN. Our data suggest that a fragment of approximately 60 kDa that co-purified with FN, with affinity to heparin and gelatin, has the arFN that controls vWF multimer size.
Palabras clave: Von Willebrand Factor , Fibronectin , Multimer Size Regulation , Heparin Affinity
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/67151
URL: https://journals.lww.com/bloodcoagulation/pages/articleviewer.aspx?year=2003&iss
Colecciones
Articulos(CCT - TANDIL)
Articulos de CTRO CIENTIFICO TECNOLOGICO CONICET - TANDIL
Articulos(IMEX)
Articulos de INST.DE MEDICINA EXPERIMENTAL
Citación
Kempfer, Ana Catalina; Farias, Cristina Elena; Amaral, María Marta; Silaf, María R.; Woods, Adriana Inés; et al.; Control of von Willebrand factor multimer size by a fibronectin-related substance; Lippincott Williams; Blood Coagulation & Fibrinolysis : An International Journal In Haemostasis And Thrombosis.; 14; 5; 7-2003; 441-448
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