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dc.contributor.author
Medawar Aguilar, Victoria
dc.contributor.author
Messina, Germán Alejandro
dc.contributor.author
Fernández Baldo, Martín Alejandro
dc.contributor.author
Raba, Julio
dc.contributor.author
Pereira, Sirley Vanesa
dc.date.available
2018-11-20T17:48:43Z
dc.date.issued
2017-01
dc.identifier.citation
Medawar Aguilar, Victoria; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro; Raba, Julio; Pereira, Sirley Vanesa; Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis; Elsevier Science; Microchemical Journal; 130; 1-2017; 436-441
dc.identifier.issn
0026-265X
dc.identifier.uri
http://hdl.handle.net/11336/64763
dc.description.abstract
The objective of this work was to develop a microfluidic immunosensor for the quantitative determination of IgG antibodies to Toxocara canis (IgG anti-T.canis), causal agent of toxocariasis. This disease is caused by accidental ingestion of infective eggs that hatch into the first portion of the intestine. Subsequently, the juvenile stages are distributed throughout the body, generating symptoms from mild to severe manifestations. The possibility of early diagnosis is of great importance, allowing proper management and treatment of patients suffering from toxocariasis. IgG anti-T.canis antibodies detection was carried out using a non-competitive immunoassay, in which excretory-secretory antigens from T. canis second-stage larvae (TES) were covalently immobilized on 3-aminopropyl-functionalized silica-nanoparticles (AP-SNs). Antibodies present in serum samples immunologically reacted with TES and then were quantified by using a second antibody labeled with cadmium selenide zinc sulfide quantum dots (CdSe-ZnS QDs). The concentration of IgG anti-T. canis antibodies present in the serum sample was measured by LIF detector, using excitation lambda at 491 nm and emission at 540 nm. The total assay time was 30 minutes, having made LIF detection in less than 1 minute. The detection limit calculated for the proposed methodology was 0.12 ng mL-1 and the coefficients of intra- and inter-assay variation were less than 6%. The results show the usefulness of the developed immunosensor for the fast determination of IgG antibodies anti T. canis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Toxocara
dc.subject
Microfluidic Immunosensor
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Laser Induced Fluorescence
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Quantumdot
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Silica Particle
dc.subject.classification
Otras Ciencias Químicas
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Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-23T17:39:58Z
dc.journal.volume
130
dc.journal.pagination
436-441
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Medawar Aguilar, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
dc.description.fil
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
dc.description.fil
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
dc.description.fil
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
dc.description.fil
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
dc.journal.title
Microchemical Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.microc.2016.10.027


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