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Artículo

High performance collection of cerebrospinal fluid in rats: evaluation of erythropoietin penetration after osmotic opening of the blood-brain barrier

Ceaglio, Natalia AnaliaIcon ; Orozco, Gustavo; Etcheverrigaray, MarinaIcon ; Mattio, Mónica CristinaIcon ; Kratje, Ricardo BertoldoIcon ; Perotti, Norma MariaIcon ; Oggero Eberhardt, Marcos RafaelIcon
Fecha de publicación: 09/2013
Editorial: Elsevier
Revista: Journal of Neuroscience Methods
ISSN: 0165-0270
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biotecnología relacionada con la Salud

Resumen

An important issue to be considered when studying a new drug for treatment of central nervous system (CNS) diseases is its ability to cross the blood–brain barrier (BBB) and distribute throughout the brain. As cerebrospinal fluid (CSF) has demonstrated to be an invaluable reservoir to study CNS availability of therapeutic proteins, we have developed an improved method for CSF sampling from the cisterna magna of rats. The technique enables the simple and rapid collection of adequate quantities (50–75 μl) of blood-free CSF, rendering a high percentage of animal survival (99%) without clinic or neurological consequences. Its success in avoiding blood contamination of CSF lays in the use of a mixture of lidocaine/ephinephrine topically injected in the rat's suboccipital area and neck. Another relevant feature of the methodology is its low cost, since the puncture device can be easily assembled with cheap and available materials and, more importantly, neither expensive stereotaxic equipment nor frame is required. The present method is demonstrated by studying the CSF pharmacokinetics of recombinant human erythropoietin (rhEPO), a well-studied therapeutic candidate for neurological diseases. Moreover, we applied this technique to evaluate a strategy of osmotic disruption of the BBB to achieve a faster delivery of rhEPO into the CNS.
Palabras clave: Cerebrospinal Fluid , Blood-Brain Barrier , Sampling Method , Osmotic Disruption , Rhepo
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/6392
URL: http://www.sciencedirect.com/science/article/pii/S0165027013002471
DOI: http://dx.doi.org/10.1016/j.jneumeth.2013.07.009
DOI: http://dx.doi.org/ 10.1016/j.jneumeth.2013.07.009
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Citación
Ceaglio, Natalia Analia; Orozco, Gustavo; Etcheverrigaray, Marina; Mattio, Mónica Cristina; Kratje, Ricardo Bertoldo; et al.; High performance collection of cerebrospinal fluid in rats: evaluation of erythropoietin penetration after osmotic opening of the blood-brain barrier; Elsevier; Journal of Neuroscience Methods; 219; 1; 9-2013; 70-75
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