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dc.contributor.author
Rodi, Pablo Marcelo
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dc.contributor.author
Maggio, Bruno
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dc.contributor.author
Bagatolli, Luis Alberto
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dc.date.available
2018-11-02T17:44:02Z
dc.date.issued
2018-02
dc.identifier.citation
Rodi, Pablo Marcelo; Maggio, Bruno; Bagatolli, Luis Alberto; Direct visualization of the lateral structure of giant vesicles composed of pseudo-binary mixtures of sulfatide, asialo-GM1 and GM1 with POPC; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1860; 2; 2-2018; 544-555
dc.identifier.issn
0005-2736
dc.identifier.uri
http://hdl.handle.net/11336/63517
dc.description.abstract
We compared the lateral structure of giant unilamellar vesicles (GUVs) composed of three pseudo binary mixtures of different glycosphingolipid (GSL), i.e. sulfatide, asialo-GM1 or GM1, with POPC. These sphingolipids possess similar hydrophobic residues but differ in the size and charge of their polar head group. Fluorescence microscopy experiments using LAURDAN and DiIC18 show coexistence of micron sized domains in a molar fraction range that depends on the nature of the GSLs. In all cases, experiments with LAURDAN show that the membrane lateral structure resembles the coexistence of solid ordered and liquid disordered phases. Notably, the overall extent of hydration measured by LAURDAN between the solid ordered and liquid disordered membrane regions show marked similarities and are independent of the size of the GSL polar head group. In addition, the maximum amount of GSL incorporated in the POPC bilayer exhibits a strong dependence on the size of the GSL polar head group following the order sulfatide > asialo-GM1 > GM1. This observation is in full harmony with previous experiments and theoretical predictions for mixtures of these GSL with glycerophospholipids. Finally, compared with previous results reported in GUVs composed of mixtures of POPC with the sphingolipids cerebroside and ceramide, we observed distinctive curvature effects at particular molar fraction regimes in the different mixtures. This suggests a pronounced effect of these GSL on the spontaneous curvature of the bilayer. This observation may be relevant in a biological context, particularly in connection with the highly curved structures found in neural cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Asialo-Gm1
dc.subject
Giant Unilamellar Vesicles
dc.subject
Gm1
dc.subject
Laurdan Gp
dc.subject
Membrane Domains
dc.subject
Membrane Hydration
dc.subject
Sphingolipids
dc.subject
Sulfatide
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Direct visualization of the lateral structure of giant vesicles composed of pseudo-binary mixtures of sulfatide, asialo-GM1 and GM1 with POPC
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-22T15:52:20Z
dc.journal.volume
1860
dc.journal.number
2
dc.journal.pagination
544-555
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Rodi, Pablo Marcelo. MEMPHYS - Center for Biomembrane Physics; Dinamarca. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.description.fil
Fil: Bagatolli, Luis Alberto. MEMPHYS - Center for Biomembrane Physics; Dinamarca. Universidad Yachay Tech; Ecuador. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Biochimica et Biophysica Acta - Biomembranes
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005273617303401
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.bbamem.2017.10.022
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