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Artículo

Direct visualization of the lateral structure of giant vesicles composed of pseudo-binary mixtures of sulfatide, asialo-GM1 and GM1 with POPC

Rodi, Pablo MarceloIcon ; Maggio, BrunoIcon ; Bagatolli, Luis AlbertoIcon
Fecha de publicación: 02/2018
Editorial: Elsevier Science
Revista: Biochimica et Biophysica Acta - Biomembranes
ISSN: 0005-2736
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

We compared the lateral structure of giant unilamellar vesicles (GUVs) composed of three pseudo binary mixtures of different glycosphingolipid (GSL), i.e. sulfatide, asialo-GM1 or GM1, with POPC. These sphingolipids possess similar hydrophobic residues but differ in the size and charge of their polar head group. Fluorescence microscopy experiments using LAURDAN and DiIC18 show coexistence of micron sized domains in a molar fraction range that depends on the nature of the GSLs. In all cases, experiments with LAURDAN show that the membrane lateral structure resembles the coexistence of solid ordered and liquid disordered phases. Notably, the overall extent of hydration measured by LAURDAN between the solid ordered and liquid disordered membrane regions show marked similarities and are independent of the size of the GSL polar head group. In addition, the maximum amount of GSL incorporated in the POPC bilayer exhibits a strong dependence on the size of the GSL polar head group following the order sulfatide > asialo-GM1 > GM1. This observation is in full harmony with previous experiments and theoretical predictions for mixtures of these GSL with glycerophospholipids. Finally, compared with previous results reported in GUVs composed of mixtures of POPC with the sphingolipids cerebroside and ceramide, we observed distinctive curvature effects at particular molar fraction regimes in the different mixtures. This suggests a pronounced effect of these GSL on the spontaneous curvature of the bilayer. This observation may be relevant in a biological context, particularly in connection with the highly curved structures found in neural cells.
Palabras clave: Asialo-Gm1 , Giant Unilamellar Vesicles , Gm1 , Laurdan Gp , Membrane Domains , Membrane Hydration , Sphingolipids , Sulfatide
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
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URI: http://hdl.handle.net/11336/63517
URL: https://www.sciencedirect.com/science/article/pii/S0005273617303401
DOI: https://doi.org/10.1016/j.bbamem.2017.10.022
Colecciones
Articulos(INIMEC - CONICET)
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Citación
Rodi, Pablo Marcelo; Maggio, Bruno; Bagatolli, Luis Alberto; Direct visualization of the lateral structure of giant vesicles composed of pseudo-binary mixtures of sulfatide, asialo-GM1 and GM1 with POPC; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1860; 2; 2-2018; 544-555
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