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dc.contributor.author Castillo, Lilian Fedra
dc.contributor.author Rivero, Ezequiel Mariano
dc.contributor.author Goffin, Vincent
dc.contributor.author Luthy, Isabel Alicia
dc.date.available 2018-10-25T16:02:26Z
dc.date.issued 2017-06
dc.identifier.citation Castillo, Lilian Fedra; Rivero, Ezequiel Mariano; Goffin, Vincent; Luthy, Isabel Alicia; Alpha2-adrenoceptor agonists trigger prolactin signaling in breast cancer cells; Elsevier Science Inc; Cellular Signalling; 34; 6-2017; 76-85
dc.identifier.issn 0898-6568
dc.identifier.uri http://hdl.handle.net/11336/63048
dc.description.abstract Breast cancer is the most frequent malignancy among women worldwide. We have described the expression of α2-adrenoceptors in breast cancer cell lines, associated with increased cell proliferation and tumor growth. A mitogenic autocrine/paracrine loop of prolactin (Prl) has been described in breast cancer cells. We hypothesized that the α2-adrenergic enhancement of proliferation could be mediated, at least in part, by this Prl loop. In both T47D and MCF-7 cell lines, the incubation with the α2-adrenergic agonist dexmedetomidine significantly increased Prl release into the culture medium (measured by the Nb2 bioassay), this effect being reversed by the α2-adrenergic antagonist rauwolscine. No change in Prl receptors (PrlR) was observed by RT-qPCR in these cell lines. In IBH-6 cells a decrease in Prl secretion was observed at the lower dexmedetomidine concentration. The signaling pathways involved in ovine Prl (oPrl) and dexmedetomidine action were also assessed. Both compounds significantly activated STAT5 and ERK in all three cell lines. In T47D and MCF-7 cell lines also AKT was activated by both Prl and dexmedetomidine. We therefore describe the STAT5 phosphorylation by an α2-adrenergic agonist, dexmedetomidine. In T47D cells, the α2-adrenergic stimulation of cell proliferation is probably mediated, at least in part, by the Prl autocrine/paracrine loop, because this effect is abrogated by the specific PrlR antagonist Δ1?9-G129R-hPrl. The implication of Prl loop describes a novel mechanism of action of this GPCR.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Science Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject CELL SIGNALING
dc.subject IBH-6
dc.subject MCF-7
dc.subject T47D
dc.subject.classification Biología Celular, Microbiología
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.subject.classification Anatomía y Morfología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.subject.classification Anatomía y Morfología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Alpha2-adrenoceptor agonists trigger prolactin signaling in breast cancer cells
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-10-23T15:50:48Z
dc.journal.volume 34
dc.journal.pagination 76-85
dc.journal.pais Estados Unidos
dc.description.fil Fil: Castillo, Lilian Fedra. Instituto Angel H. Roffo; Argentina
dc.description.fil Fil: Rivero, Ezequiel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Goffin, Vincent. Inserm; Francia. Universite de Paris; Francia
dc.description.fil Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.journal.title Cellular Signalling
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0898656817300724
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.cellsig.2017.03.003
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/pmid/28302567
dc.conicet.fuente Elsevier


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)