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Artículo

Alpha2-adrenoceptor agonists trigger prolactin signaling in breast cancer cells

Castillo, Lilian FedraIcon ; Rivero, Ezequiel MarianoIcon ; Goffin, Vincent; Luthy, Isabel AliciaIcon
Fecha de publicación: 06/2017
Editorial: Elsevier Science Inc
Revista: Cellular Signalling
ISSN: 0898-6568
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología; Anatomía y Morfología; Anatomía y Morfología

Resumen

Breast cancer is the most frequent malignancy among women worldwide. We have described the expression of α2-adrenoceptors in breast cancer cell lines, associated with increased cell proliferation and tumor growth. A mitogenic autocrine/paracrine loop of prolactin (Prl) has been described in breast cancer cells. We hypothesized that the α2-adrenergic enhancement of proliferation could be mediated, at least in part, by this Prl loop. In both T47D and MCF-7 cell lines, the incubation with the α2-adrenergic agonist dexmedetomidine significantly increased Prl release into the culture medium (measured by the Nb2 bioassay), this effect being reversed by the α2-adrenergic antagonist rauwolscine. No change in Prl receptors (PrlR) was observed by RT-qPCR in these cell lines. In IBH-6 cells a decrease in Prl secretion was observed at the lower dexmedetomidine concentration. The signaling pathways involved in ovine Prl (oPrl) and dexmedetomidine action were also assessed. Both compounds significantly activated STAT5 and ERK in all three cell lines. In T47D and MCF-7 cell lines also AKT was activated by both Prl and dexmedetomidine. We therefore describe the STAT5 phosphorylation by an α2-adrenergic agonist, dexmedetomidine. In T47D cells, the α2-adrenergic stimulation of cell proliferation is probably mediated, at least in part, by the Prl autocrine/paracrine loop, because this effect is abrogated by the specific PrlR antagonist Δ1?9-G129R-hPrl. The implication of Prl loop describes a novel mechanism of action of this GPCR.
Palabras clave: Cell Signaling , Ibh-6 , Mcf-7 , T47d
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/63048
URL: https://www.sciencedirect.com/science/article/pii/S0898656817300724
DOI: http://dx.doi.org/10.1016/j.cellsig.2017.03.003
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Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Castillo, Lilian Fedra; Rivero, Ezequiel Mariano; Goffin, Vincent; Luthy, Isabel Alicia; Alpha2-adrenoceptor agonists trigger prolactin signaling in breast cancer cells; Elsevier Science Inc; Cellular Signalling; 34; 6-2017; 76-85
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