Artículo
The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles
Alarcon, Emilio I.; Udekwu, Klas; Skog, Mårten; Pacioni, Natalia Lorena
; Stamplecoskie, Kevin G.; González Béjar, María; Polisetti, Naresh; Wickham, Abeni; Richter Dahlfors, Agneta; Griffith, May; Scaiano, Juan Cesar
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Fecha de publicación:
06/2012
Editorial:
Elsevier
Revista:
Biomaterials
ISSN:
0142-9612
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Spherical 3.5 nm diameter silver nanoparticles (AgNP) stabilized in type I collagen (. AgNP@collagen) were prepared in minutes (5-15 min) at room temperature by a photochemical method initiated by UVA irradiation of a water-soluble non-toxic benzoin. This biocomposite was examined to evaluate its biocompatibility and its anti-bacterial properties and showed remarkable properties. Thus, while keratinocytes and fibroblasts were not affected by . AgNP@collagen, it was bactericidal against . Bacillus megaterium and . E. coli but only bacteriostatic against . S. . epidermidis. In particular, the bactericidal properties displayed by . AgNP@collagen were proven to be due to AgNP in . AgNP@collagen, rather than to released silver ions, since equimolar concentrations of Ag are about four times less active than . AgNP@collagen based on total Ag content. This new biocomposite was stable over a remarkable range of NaCl, phosphate, and 2-(N-morpholino)ethanesulfonic acid concentrations and for over one month at 4 °C. Circular dichroism studies show that the conformation of collagen in . AgNP@collagen remains intact. Finally, we have compared the properties of . AgNP@collagen with a similar biocomposite prepared using α-poly-. l-Lysine and also with citrate stabilized AgNP; neither of these materials showed comparable biocompatibility, stability, or anti-bacterial activity. © 2012 Elsevier Ltd.
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Articulos(INFIQC)
Articulos de INST.DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Articulos de INST.DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Citación
Alarcon, Emilio I.; Udekwu, Klas; Skog, Mårten; Pacioni, Natalia Lorena; Stamplecoskie, Kevin G.; et al.; The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles; Elsevier; Biomaterials; 33; 19; 6-2012; 4947-4956
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