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Artículo

Yersinia enterocolitica YopH-deficient strain activates neutrophil recruitment to Peyer's patches and promotes clearance of the virulent strain

Dave, Mabel NoemíIcon ; Silva, Juan EduardoIcon ; Eliçabe, Ricardo JavierIcon ; Jeréz, María BelénIcon ; Filippa, Veronica PalmiraIcon ; Gorlino, Carolina VirginiaIcon ; Autenrieth, Stella; Autenrieth, Ingo B.; Di Genaro, Maria SilviaIcon
Fecha de publicación: 08/2016
Editorial: American Society for Microbiology
Revista: Infection and Immunity
ISSN: 0019-9567
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias de la Salud

Resumen

Yersinia enterocolitica evades the immune response by injecting Yersinia outer proteins (Yops) into the cytosol of host cells. YopH is a tyrosine phosphatase critical for Yersinia virulence. However, the mucosal immune mechanisms subverted by YopH during in vivo orogastric infection with Y. enterocolitica remain elusive. The results of this study revealed neutrophil recruitment to Peyer's patches (PP) after infection with a YopH-deficient mutant strain (Y. enterocolitica ΔyopH). While the Y. enterocolitica wild-type (WT) strain in PP induced the major neutrophil chemoattractant CXCL1 mRNA and protein levels, infection with the Y. enterocolitica ΔyopH mutant strain exhibited a higher expression of the CXCL1 receptor, CXCR2, in blood neutrophils, leading to efficient neutrophil recruitment to the PP. In contrast, migration of neutrophils into PP was impaired upon infection with Y. enterocolitica WT strain. In vitro infection of blood neutrophils revealed the involvement of YopH in CXCR2 expression. Depletion of neutrophils during Y. enterocolitica ΔyopH infection raised the bacterial load in PP. Moreover, the clearance of WT Y. enterocolitica was improved when an equal mixture of Y. enterocolitica WT and Y. enterocolitica ΔyopH strains was used in infecting the mice. This study indicates that Y. enterocolitica prevents early neutrophil recruitment in the intestine and that the effector protein YopH plays an important role in the immune evasion mechanism. The findings highlight the potential use of the Y. enterocolitica YopH-deficient strain as an oral vaccine carrier.
Palabras clave: Yersinia Enterocolitica , Mucosa , Neutrophils , Cxcr2
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/60784
URL: https://iai.asm.org/content/84/11/3172
DOI: http://dx.doi.org/10.1128/IAI.00568-16
Colecciones
Articulos(CCT - SAN LUIS)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Articulos(IMIBIO-SL)
Articulos de INST. MULTIDICIPLINARIO DE INV. BIO. DE SAN LUIS
Citación
Dave, Mabel Noemí; Silva, Juan Eduardo; Eliçabe, Ricardo Javier; Jeréz, María Belén; Filippa, Veronica Palmira; et al.; Yersinia enterocolitica YopH-deficient strain activates neutrophil recruitment to Peyer's patches and promotes clearance of the virulent strain; American Society for Microbiology; Infection and Immunity; 84; 11; 8-2016; 3172-3181
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