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Artículo

Cobalamin production by Lactobacillus coryniformis: Biochemical identification of the synthetized corrinoid and genomic analysis of the biosynthetic cluster

Torres, Andrea CarolinaIcon ; Vannini, María VerónicaIcon ; Bonacina, JulietaIcon ; Font, Graciela MariaIcon ; Saavedra, Maria LucilaIcon ; Taranto, Maria PiaIcon
Fecha de publicación: 13/10/2016
Editorial: BioMed Central
Revista: BMC Microbiology
ISSN: 1471-2180
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas; Ciencias de la Computación

Resumen

Background: Despite the fact that most vitamins are present in a variety of foods, malnutrition, unbalanced diets or insufficient intake of foods are still the cause of vitamin deficiencies in humans in some countries. Vitamin B12 (Cobalamin) is a complex compound that is only naturally produced by bacteria and archea. It has been reported that certain strains belonging to lactic acid bacteria group are capable of synthesized water-soluble vitamins such as those included in the B-group, as vitamin B12. In this context, the goal of the present paper was to evaluate and characterize the production of vitamin B12 in Lactobacillus coryniformis CRL 1001, a heterofermentative strain isolated from silage. Results: Cell extract of L. coryniformis CRL 1001, isolated from silage, is able to correct the coenzyme B12 requirement of Salmonella enterica serovar Typhimurium AR 2680 in minimal medium. The chemical characterization of the corrinoid-like molecule isolated from CRL 1001 cell extract using HPLC and mass spectrometry is reported. The majority of the corrinoid produced by this strain has adenine like Coα-ligand instead 5,6-dimethylbenzimidazole. Genomic studies revealed the presence of the complete machinery of the anaerobic biosynthesis pathway of coenzyme B12. The detected genes encode all proteins for the corrin ring biosynthesis and for the binding of upper (β) and lower (α) ligands in one continuous stretch of the chromosome. Conclusions: The results here described show for the first time that L. coryniformis subsp. coryniformis CRL 1001 is able to produce pseudocobalamin containing adenine instead of 5,6-dimethlbenzimidazole in the Coα-ligand. Genomic analysis allowed the identification and characterization of the complete de novo biosynthetic pathway of the corrinoid produced by the CRL 1001 strain.
Palabras clave: Cobalamin Production , Coenzyme B12 Gene Cluster Organization , Lactobacillus
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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URI: http://hdl.handle.net/11336/59214
DOI: http://dx.doi.org/10.1186/s12866-016-0854-9
URL: https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0854-9
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Articulos(CERELA) [925]
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Citación
Torres, Andrea Carolina; Vannini, María Verónica; Bonacina, Julieta; Font, Graciela Maria; Saavedra, Maria Lucila; et al.; Cobalamin production by Lactobacillus coryniformis: Biochemical identification of the synthetized corrinoid and genomic analysis of the biosynthetic cluster; BioMed Central; BMC Microbiology; 16; 1; 13-10-2016; 1-9;240-240
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