Mostrar el registro sencillo del ítem
dc.contributor.author
Scarcella, Silvana Andrea
dc.contributor.author
Hanna, R. E. B.
dc.contributor.author
Brennan, G. P.
dc.contributor.author
Solana, Hugo Daniel
dc.contributor.author
Fairweather, I.
dc.date.available
2018-09-05T19:23:26Z
dc.date.issued
2016-01
dc.identifier.citation
Scarcella, Silvana Andrea; Hanna, R. E. B.; Brennan, G. P.; Solana, Hugo Daniel; Fairweather, I.; Fasciola hepatica: Histological changes in the somatic and reproductive tissues of liver fluke following closantel treatment of experimentally-infected sheep; Elsevier Science; Veterinary Parasitology; 215; 1-2016; 38-47
dc.identifier.issn
0304-4017
dc.identifier.uri
http://hdl.handle.net/11336/58431
dc.description.abstract
Lambs infected with the Cullompton isolate of Fasciola hepatica were treated orally or subcutaneously with 10 mg/kg of closantel at 16 weeks post-infection. Adult flukes were recovered from the liver of individual animals at 12 h, 24 h, or 36 h post-treatment. The flukes were processed for histological analysis. In general, degenerative changes in the reproductive and somatic tissues were progressive, and were most marked in flukes exposed to closantel in vivo for 36 h. However, flukes from a 12 h subcutaneously-treated lamb showed marked deterioration of the testis, possibly because a portion of the dose has been delivered intravenously. Fewer intact eggs were seen in the uterus of flukes exposed to closantel for longer times (whether administered subcutaneously or orally to the host). The most conspicuous closantel-induced effect in flukes from treated hosts was progressive damage to the tegumental syncytium. While the flukes from 24 h-treated hosts showed relatively minor damage to limited areas of the syncytium, towards the posterior end, the flukes from 36 h-treated hosts (and flukes from the lamb that putatively received intravenous dosage) had lost large areas of the surface syncytium from the posterior end and dorsal surface, although the syncytium over the anterior end and the anterior ventral surface was largely spared. In areas where the syncytium had sloughed, the underlying structures such as the vitelline follicles, gut profiles and testis profiles, showed marked degeneration and breakdown. Other changes included cell depletion and early stage apoptosis in the testis, ovary and vitelline follicles. This study establishes a model for histological changes in closantel-sensitive F. hepatica exposed to closantel in vivo.Histopathological studies could be complementary to the efficacy controlled test for for closantel resistance in fluke populations.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Closantel in Vivo
dc.subject
Fasciola Hepatica
dc.subject
Fluke Resistance Diagnosis
dc.subject
Liver Fluke
dc.subject
Post-Treatment Fluke Histology
dc.subject
Sheep
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Fasciola hepatica: Histological changes in the somatic and reproductive tissues of liver fluke following closantel treatment of experimentally-infected sheep
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-09-05T15:53:51Z
dc.journal.volume
215
dc.journal.pagination
38-47
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Scarcella, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
dc.description.fil
Fil: Hanna, R. E. B.. Agri-Food and Biosciences Institute; Reino Unido
dc.description.fil
Fil: Brennan, G. P.. The Queens University of Belfast; Irlanda
dc.description.fil
Fil: Solana, Hugo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
dc.description.fil
Fil: Fairweather, I.. The Queens University of Belfast; Irlanda
dc.journal.title
Veterinary Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304401715300662
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vetpar.2015.10.029
Archivos asociados