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dc.contributor.author
Verna, Andrea Elizabeth
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dc.contributor.author
Franceschi, Valentina
dc.contributor.author
Tebaldi, Giulia
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Macchi, Francesca
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Menozzi, Valentina
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Pastori, Claudia
dc.contributor.author
Lopalco, Lucia
dc.contributor.author
Ottonello, Simone
dc.contributor.author
Cavirani, Sandro
dc.contributor.author
Donofrio, Gaetano
dc.date.available
2018-08-14T19:32:56Z
dc.date.issued
2017-10
dc.identifier.citation
Verna, Andrea Elizabeth; Franceschi, Valentina; Tebaldi, Giulia; Macchi, Francesca; Menozzi, Valentina; et al.; Induction of antihuman C-C chemokine receptor type 5 antibodies by a bovine herpesvirus type-4 based vector; Frontiers Media S.A.; Frontiers in Immunology; 8; OCT; 10-2017; 1-10
dc.identifier.issn
1664-3224
dc.identifier.uri
http://hdl.handle.net/11336/55482
dc.description.abstract
Bovine herpesvirus 4 (BoHV-4) is a promising vector for the delivery and intracellular expression of recombinant antigens and can thus be considered as a new prototype vaccine formulation system. An interesting, and actively pursued, antigen in the context of human immunodeficiency virus (HIV) infection prophylaxis (and therapy) is the C-C chemokine receptor type 5 (CCR5) co-receptor, whose blockage by specific antibodies has been shown to inhibit both viral entry and cell-to-cell transmission of the virus. Building on our previous work on the BoHV-4 vector system, we have engineered and tested a replication-competent derivative of BoHV-4 (BoHV-4-CMV-hCCR5?TK) bearing a human CCR5 (hCCR5) expression cassette. We show here that CCR5 is indeed expressed at high levels in multiple types of BoHV-4-CMV-hCCR5?TK-infected cells. More importantly, two intravenous inoculations of CCR5-expressing BoHV-4 virions into rabbits led to the production of anti-CCR5 antibodies capable of reacting with the CCR5 receptor exposed on the surface of HEK293T cells through specific recognition of the amino-terminal region (aa 14-34) of the protein. Given the growing interest for anti-CCR5 immunization as an HIV control strategy and the many advantages of virus-based immunogen formulations (especially for poorly immunogenic or self-antigens), the results reported in this study provide preliminary validation of BoHV-4 as a safe viral vector suitable for CCR5 vaccination.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media S.A.
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Antihuman Ccr5 Antibodies
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Bovine Herpesvirus 4
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Gene Delivery Vector
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Human Ccr5
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Recombinant Virus
dc.subject.classification
Otras Ciencias Veterinarias
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dc.subject.classification
Ciencias Veterinarias
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Induction of antihuman C-C chemokine receptor type 5 antibodies by a bovine herpesvirus type-4 based vector
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-08-13T17:24:46Z
dc.journal.volume
8
dc.journal.number
OCT
dc.journal.pagination
1-10
dc.journal.pais
Austria
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dc.journal.ciudad
Viena
dc.description.fil
Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Università di Parma; Italia
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Fil: Franceschi, Valentina. Università di Parma; Italia
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Fil: Tebaldi, Giulia. Università di Parma; Italia
dc.description.fil
Fil: Macchi, Francesca. Università di Parma; Italia
dc.description.fil
Fil: Menozzi, Valentina. Università di Parma; Italia
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Fil: Pastori, Claudia. San Raffaele Scientific Institute; Italia
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Fil: Lopalco, Lucia. San Raffaele Scientific Institute; Italia
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Fil: Ottonello, Simone. Università di Parma; Italia
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Fil: Cavirani, Sandro. Università di Parma; Italia
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Fil: Donofrio, Gaetano. Università di Parma; Italia
dc.journal.title
Frontiers in Immunology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.3389/fimmu.2017.01402
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2017.01402/full
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