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Artículo

Induction of antihuman C-C chemokine receptor type 5 antibodies by a bovine herpesvirus type-4 based vector

Verna, Andrea ElizabethIcon ; Franceschi, Valentina; Tebaldi, Giulia; Macchi, Francesca; Menozzi, Valentina; Pastori, Claudia; Lopalco, Lucia; Ottonello, Simone; Cavirani, Sandro; Donofrio, Gaetano
Fecha de publicación: 10/2017
Editorial: Frontiers Media S.A.
Revista: Frontiers in Immunology
ISSN: 1664-3224
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Veterinarias

Resumen

Bovine herpesvirus 4 (BoHV-4) is a promising vector for the delivery and intracellular expression of recombinant antigens and can thus be considered as a new prototype vaccine formulation system. An interesting, and actively pursued, antigen in the context of human immunodeficiency virus (HIV) infection prophylaxis (and therapy) is the C-C chemokine receptor type 5 (CCR5) co-receptor, whose blockage by specific antibodies has been shown to inhibit both viral entry and cell-to-cell transmission of the virus. Building on our previous work on the BoHV-4 vector system, we have engineered and tested a replication-competent derivative of BoHV-4 (BoHV-4-CMV-hCCR5?TK) bearing a human CCR5 (hCCR5) expression cassette. We show here that CCR5 is indeed expressed at high levels in multiple types of BoHV-4-CMV-hCCR5?TK-infected cells. More importantly, two intravenous inoculations of CCR5-expressing BoHV-4 virions into rabbits led to the production of anti-CCR5 antibodies capable of reacting with the CCR5 receptor exposed on the surface of HEK293T cells through specific recognition of the amino-terminal region (aa 14-34) of the protein. Given the growing interest for anti-CCR5 immunization as an HIV control strategy and the many advantages of virus-based immunogen formulations (especially for poorly immunogenic or self-antigens), the results reported in this study provide preliminary validation of BoHV-4 as a safe viral vector suitable for CCR5 vaccination.
Palabras clave: Antihuman Ccr5 Antibodies , Bovine Herpesvirus 4 , Gene Delivery Vector , Human Ccr5 , Recombinant Virus
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/55482
DOI: https://dx.doi.org/10.3389/fimmu.2017.01402
URL: https://www.frontiersin.org/articles/10.3389/fimmu.2017.01402/full
Colecciones
Articulos(CCT - MAR DEL PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MAR DEL PLATA
Citación
Verna, Andrea Elizabeth; Franceschi, Valentina; Tebaldi, Giulia; Macchi, Francesca; Menozzi, Valentina; et al.; Induction of antihuman C-C chemokine receptor type 5 antibodies by a bovine herpesvirus type-4 based vector; Frontiers Media S.A.; Frontiers in Immunology; 8; OCT; 10-2017; 1-10
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