Artículo
Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests
Mañas, Fernando Javier
; Peralta, Laura; Raviolo, Jose Maria
; Garcia Ovando, Hugo; Weyers, Irma Alicia; Ugnia, Laura Inés; Gonzalez Cid, Marcela Beatriz
; Larripa, Irene Beatriz
; Gorla, Nora Bibiana Maria
Fecha de publicación:
07/2009
Editorial:
Elsevier Science
Revista:
Environmental Toxicology and Pharmacology
ISSN:
1382-6689
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
It was evaluated the genotoxicity of glyphosate which up to now has heterogeneous results. The comet assay was performed in Hep-2 cells. The level of DNA damage in the control group (5.42 ± 1.83 arbitrary units) for tail moment (TM) measurements has shown a significant increase (p < 0.01) with glyphosate at a range concentration from 3.00 to 7.50 mM. In the chromosome aberrations (CA) test in human lymphocytes the herbicide (0.20-6.00 mM) showed no significant effects in comparison with the control group. In vivo, the micronucleus test (MNT) was evaluated in mice at three doses rendering statistical significant increases at 400 mg/kg (13.0 ± 3.08 micronucleated erythrocytes/1000 cells, p < 0.01). In the present study glyphosate was genotoxic in the comet assay in Hep-2 cells and in the MNT test at 400 mg/kg in mice. Thiobarbituric acid reactive substances (TBARs) levels, superoxide dismutase (SOD) and catalase (CAT) activities were quantified in their organs. The results showed an increase in these enzyme activities. © 2009 Elsevier B.V. All rights reserved.
Palabras clave:
Catalase
,
Chromosome Aberrations
,
Comet Assay
,
Glyphosate
,
Micronucleus
,
Sod
,
Tbars
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IMEX)
Articulos de INST.DE MEDICINA EXPERIMENTAL
Articulos de INST.DE MEDICINA EXPERIMENTAL
Citación
Mañas, Fernando Javier; Peralta, Laura; Raviolo, Jose Maria; Garcia Ovando, Hugo; Weyers, Irma Alicia; et al.; Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests; Elsevier Science; Environmental Toxicology and Pharmacology; 28; 1; 7-2009; 37-41
Compartir
Altmétricas