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dc.contributor.author
Ortiz, Gastón Ezequiel  
dc.contributor.author
Alberto, Edgardo Omar  
dc.contributor.author
Blasco, Martín  
dc.date.available
2018-08-03T20:52:03Z  
dc.date.issued
2016-01  
dc.identifier.citation
Ortiz, Gastón Ezequiel; Alberto, Edgardo Omar; Blasco, Martín; Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30; Trade science inc.; BioTechnology: An Indian Journal; 12; 1; 1-2016; 39-44  
dc.identifier.issn
0974-7435  
dc.identifier.uri
http://hdl.handle.net/11336/54189  
dc.description.abstract
In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functional study of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vector was employed to transform T. reesei Rut-C30. The transformants obtained were evaluated by means of fluorescence microscopy, fluorometry, dot blot and Western blot analysis. The low levels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Trade science inc.  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Trichoderma Reesei  
dc.subject
Toxa Promoter  
dc.subject
Recombinant Protein Expres-Sion  
dc.subject
Weak Promoter.  
dc.subject.classification
Biotecnología Industrial  
dc.subject.classification
Biotecnología Industrial  
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-08-03T14:17:32Z  
dc.journal.volume
12  
dc.journal.number
1  
dc.journal.pagination
39-44  
dc.journal.pais
India  
dc.description.fil
Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina  
dc.description.fil
Fil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina  
dc.description.fil
Fil: Blasco, Martín. Instituto Nacional de Tecnología Industrial; Argentina  
dc.journal.title
BioTechnology: An Indian Journal  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.tsijournals.com/abstract/functional-evaluation-of-toxa-promoter-in-trichoderma-reeseirutc30-2219.html  

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