Artículo

Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30

Ortiz, Gastón EzequielIcon ; Alberto, Edgardo OmarIcon ; Blasco, MartínIcon
Fecha de publicación: 01/2016
Editorial: Trade science inc.
Revista: BioTechnology: An Indian Journal
ISSN: 0974-7435
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functional study of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vector was employed to transform T. reesei Rut-C30. The transformants obtained were evaluated by means of fluorescence microscopy, fluorometry, dot blot and Western blot analysis. The low levels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30
Palabras clave: Trichoderma Reesei , Toxa Promoter , Recombinant Protein Expres-Sion , Weak Promoter.
 
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Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/54189
URL: http://www.tsijournals.com/abstract/functional-evaluation-of-toxa-promoter-in-tr
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Ortiz, Gastón Ezequiel; Alberto, Edgardo Omar; Blasco, Martín; Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30; Trade science inc.; BioTechnology: An Indian Journal; 12; 1; 1-2016; 39-44
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