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dc.contributor.author
Caballero, Marina
dc.contributor.author
Alonso, Andrés Mariano
dc.contributor.author
Deng, Bin
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Attias, Marcia
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De Souza, Wanderley
dc.contributor.author
Corvi, Maria Martha
dc.date.available
2018-07-31T18:22:48Z
dc.date.issued
2016-04
dc.identifier.citation
Caballero, Marina; Alonso, Andrés Mariano; Deng, Bin; Attias, Marcia; De Souza, Wanderley; et al.; Identification of new palmitoylated proteins in Toxoplasma gondii; Elsevier Science; Biochimica Et Biophysica Acta-proteins And Proteomics; 1864; 4; 4-2016; 400-408
dc.identifier.issn
1570-9639
dc.identifier.uri
http://hdl.handle.net/11336/53623
dc.description.abstract
Protein palmitoylation has been shown to be an important post-translational modification in eukaryotic cells. This modification alters the localization and/or the function of the targeted protein. In recent years, protein palmitoylation has risen in importance in apicomplexan parasites as well. In Toxoplasma gondii, some proteins have been reported to be modified by palmitate. With the development of new techniques that allow the isolation of palmitoylated proteins, this significant post-translational modification has begun to be studied in more detail in T. gondii. Here we describe the palmitoylome of the tachyzoite stage of T. gondii using a combination of the acyl-biotin exchange chemistry method and mass spectrometry analysis. We identified 401 proteins found in multiple cellular compartments, with a wide range of functions that vary from metabolic processes, gliding and host-cell invasion to even regulation of transcription and translation. Besides, we found that more rhoptry proteins than the ones already described for Toxoplasma are palmitoylated, suggesting an important role for this modification in the invasion mechanism of the host-cell. This study documents that protein palmitoylation is a common modification in T. gondii that could have an impact on different cellular processes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Acyl-Biotin Exchange
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Host-Cell Invasion
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Palmitoylome
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Protein Identification
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Rhoptry
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Toxoplasma Gondii
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Identification of new palmitoylated proteins in Toxoplasma gondii
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-31T17:22:26Z
dc.journal.volume
1864
dc.journal.number
4
dc.journal.pagination
400-408
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Caballero, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
dc.description.fil
Fil: Alonso, Andrés Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
dc.description.fil
Fil: Deng, Bin. University of Vermont; Estados Unidos
dc.description.fil
Fil: Attias, Marcia. Universidade Federal do Rio de Janeiro; Brasil
dc.description.fil
Fil: De Souza, Wanderley. Universidade Federal do Rio de Janeiro; Brasil
dc.description.fil
Fil: Corvi, Maria Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
dc.journal.title
Biochimica Et Biophysica Acta-proteins And Proteomics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbapap.2016.01.010
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1570963916300024
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857766/
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