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dc.contributor.author
Campos, Débora A.
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Woitovich Valetti, Nadia
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Oliveira, Ana
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Pastrana Castro, Lorenzo M.
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Teixeira, José A.
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Pintado, Maria Manuela
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Picó, Guillermo Alfredo
dc.date.available
2018-06-27T18:00:12Z
dc.date.issued
2017-03
dc.identifier.citation
Campos, Débora A.; Woitovich Valetti, Nadia; Oliveira, Ana; Pastrana Castro, Lorenzo M.; Teixeira, José A.; et al.; Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan; Elsevier; Process Biochemistry; 54; 3-2017; 156-161
dc.identifier.issn
1359-5113
dc.identifier.uri
http://hdl.handle.net/11336/50282
dc.description.abstract
The main objective of this work was to investigate for the first time the molecular mechanism of complex formation between bromelain (a positively charged enzyme) and carrageenan (a natural strong polyelectrolyte, negatively charged) using spectroscopy techniques and thermodynamic approaches. The Bromelain-Carrageenan complex showed a maximal non-solubility at pH around 5.1. The solubility was dependent on pH and ionic strength of the medium. To re-dissolve the formed complex, the pH was changed and 500 mM of NaCl was added to the initial solution, proving the columbic mechanism for the formation of non-soluble complex. The formation of the carrageenan-bromelain complex increased in 8 °C the enzyme thermal stability, while its biological activity was not modified. The amount of total enzyme recovered in solution after precipitation with around 0.08% w/v of carrageenan was 85–90%.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bromelain
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Carrageenan
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Enzyme Mechanism
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Polyelectrolytes
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Protein Complex
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Biotecnología Industrial
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-26T22:33:28Z
dc.journal.volume
54
dc.journal.pagination
156-161
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Campos, Débora A.. Universidade Católica Portuguesa; Portugal
dc.description.fil
Fil: Woitovich Valetti, Nadia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
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Fil: Oliveira, Ana. Universidade Católica Portuguesa; Portugal
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Fil: Pastrana Castro, Lorenzo M.. International Iberian Nanotechnology Laboratory; Portugal
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Fil: Teixeira, José A.. Universidade do Minho; Portugal
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Fil: Pintado, Maria Manuela. Universidade Católica Portuguesa; Portugal
dc.description.fil
Fil: Picó, Guillermo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
dc.journal.title
Process Biochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.procbio.2016.12.014
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1359511316305979
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