Artículo
Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan
Campos, Débora A.; Woitovich Valetti, Nadia
; Oliveira, Ana; Pastrana Castro, Lorenzo M.; Teixeira, José A.; Pintado, Maria Manuela; Picó, Guillermo Alfredo
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
03/2017
Editorial:
Elsevier
Revista:
Process Biochemistry
ISSN:
1359-5113
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The main objective of this work was to investigate for the first time the molecular mechanism of complex formation between bromelain (a positively charged enzyme) and carrageenan (a natural strong polyelectrolyte, negatively charged) using spectroscopy techniques and thermodynamic approaches. The Bromelain-Carrageenan complex showed a maximal non-solubility at pH around 5.1. The solubility was dependent on pH and ionic strength of the medium. To re-dissolve the formed complex, the pH was changed and 500 mM of NaCl was added to the initial solution, proving the columbic mechanism for the formation of non-soluble complex. The formation of the carrageenan-bromelain complex increased in 8 °C the enzyme thermal stability, while its biological activity was not modified. The amount of total enzyme recovered in solution after precipitation with around 0.08% w/v of carrageenan was 85–90%.
Palabras clave:
Bromelain
,
Carrageenan
,
Enzyme Mechanism
,
Polyelectrolytes
,
Protein Complex
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Identificadores
Colecciones
Articulos(IPROBYQ)
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Articulos de INST. DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Citación
Campos, Débora A.; Woitovich Valetti, Nadia; Oliveira, Ana; Pastrana Castro, Lorenzo M.; Teixeira, José A.; et al.; Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan; Elsevier; Process Biochemistry; 54; 3-2017; 156-161
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